摘要
为了探究羊茅属种质芽期对逆境的胁迫能力,以沟羊茅新品系(FV)与紫羊茅常用品种传奇(CQ)、草原(CY)、梦神(MS)、北方(BF)、玉山(YS)和多花(DH)为材料,采用不同浓度PEG-6000(0、5％、10％、15％、20％、25％)模拟干旱胁迫,在芽期测定了发芽率、发芽势、活力指数、胚根长、胚芽长、根芽比等萌发指标,分析干旱对其种子萌发的影响,并结合主成分分析、隶属函数分析、聚类分析等对其抗旱性进行评价.结果表明,高浓度PEG(20％～25％)显著降低了羊茅属种质的发芽势、发芽率、发芽指数、活力指数、胚根长、胚根长及根芽比,而5％～15％PEG则因种质的差异而变化趋势有所不同,其中0～10％PEG对FV、BF、YS的发芽率、发芽势、活力指数及发芽指数具有显著的引发作用;综合评价表明,主成分分析将7个相对单项性状指标降维成2个新的综合指标,累积贡献率达85.06％;以新合成的综合指标为依据,采用隶属函数法结合聚类分析,将7份羊茅属种质分为三类,其中,FV、CQ和BF的抗旱能力较强,CY及YS的抗旱能力一般,而MS、DH的抗旱能力较弱.
Abstract
In order to explore the stress ability of Fescue germplasm to stress during the bud stage,drought stress was simulated with different concentrations of PEG-6000(0,5％,10％,15％,20％,25％)using Festuca valesiaca,a new strain(FV)and common varieties of Festuca rubra Legend(CQ),Grassland(CY),Morpheus(MS),Northern(BF),Yushan(YS)and Duohua(DH)as mate-rials.Germination rate,germination potential,vitality index,radicle length,plumule length,radicle/plumule and other germination indexes were measured at the germination stage.The effects of drought on seed germination were analyzed.The drought resistance was evaluated by principal compo-nent analysis,membership function analysis and cluster analysis.The results showed that the high concentration of PEG(20％-25％)significantly decreased the germination potential,germination rate,germination index,vitality index,radicle length,radicle length and root to shoot ratio of Fescue germplasms,while the difference of 5％-15％PEG was different with different germplasms.Among them,0-10％PEG had an initiation effect on FV,BF and YS germination indexes.Comprehensive evaluation indicated that by principal component analysis,7 relative single trait indexes were trans-formed into 2 new comprehensive indexes,and the cumulative contribution rate was 85.06％.Accord-ing to the newly synthesized index,7 Fescue germpalsms were divided into 3 categories by member-ship function method combined with cluster analysis.Among them,FV,CQ and BF had strong drought resistance,CY and YS had average drought resistance,and MS and DH had weak drought resistance.
基金项目
新疆维吾尔自治区重点研发计划(2023B02031-1)
新疆维吾尔自治区高等学校基本科研业务费专项(XJEDU2022J006)
维普
万方数据