首页|7份羊茅属种质芽期抗旱性评价

7份羊茅属种质芽期抗旱性评价

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为了探究羊茅属种质芽期对逆境的胁迫能力,以沟羊茅新品系(FV)与紫羊茅常用品种传奇(CQ)、草原(CY)、梦神(MS)、北方(BF)、玉山(YS)和多花(DH)为材料,采用不同浓度PEG-6000(0、5%、10%、15%、20%、25%)模拟干旱胁迫,在芽期测定了发芽率、发芽势、活力指数、胚根长、胚芽长、根芽比等萌发指标,分析干旱对其种子萌发的影响,并结合主成分分析、隶属函数分析、聚类分析等对其抗旱性进行评价.结果表明,高浓度PEG(20%~25%)显著降低了羊茅属种质的发芽势、发芽率、发芽指数、活力指数、胚根长、胚根长及根芽比,而5%~15%PEG则因种质的差异而变化趋势有所不同,其中0~10%PEG对FV、BF、YS的发芽率、发芽势、活力指数及发芽指数具有显著的引发作用;综合评价表明,主成分分析将7个相对单项性状指标降维成2个新的综合指标,累积贡献率达85.06%;以新合成的综合指标为依据,采用隶属函数法结合聚类分析,将7份羊茅属种质分为三类,其中,FV、CQ和BF的抗旱能力较强,CY及YS的抗旱能力一般,而MS、DH的抗旱能力较弱.
Evaluation of Drought Resistance of 7 Fescue Germplasms at Bud Stage
In order to explore the stress ability of Fescue germplasm to stress during the bud stage,drought stress was simulated with different concentrations of PEG-6000(0,5%,10%,15%,20%,25%)using Festuca valesiaca,a new strain(FV)and common varieties of Festuca rubra Legend(CQ),Grassland(CY),Morpheus(MS),Northern(BF),Yushan(YS)and Duohua(DH)as mate-rials.Germination rate,germination potential,vitality index,radicle length,plumule length,radicle/plumule and other germination indexes were measured at the germination stage.The effects of drought on seed germination were analyzed.The drought resistance was evaluated by principal compo-nent analysis,membership function analysis and cluster analysis.The results showed that the high concentration of PEG(20%-25%)significantly decreased the germination potential,germination rate,germination index,vitality index,radicle length,radicle length and root to shoot ratio of Fescue germplasms,while the difference of 5%-15%PEG was different with different germplasms.Among them,0-10%PEG had an initiation effect on FV,BF and YS germination indexes.Comprehensive evaluation indicated that by principal component analysis,7 relative single trait indexes were trans-formed into 2 new comprehensive indexes,and the cumulative contribution rate was 85.06%.Accord-ing to the newly synthesized index,7 Fescue germpalsms were divided into 3 categories by member-ship function method combined with cluster analysis.Among them,FV,CQ and BF had strong drought resistance,CY and YS had average drought resistance,and MS and DH had weak drought resistance.

Festucabud stagedrought resistanceprinciple component analysismembership function methodPEG stress

周晨烨、李培英、孙宗玖、张勇娟、于冰洁、郑丽、周磊、许莹月、李有政

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新疆农业大学草业学院,乌鲁木齐 830052

新疆草地资源与生态重点实验室,乌鲁木齐 830052

西部干旱荒漠区草地资源与生态教育部重点实验室,乌鲁木齐 830052

乌鲁木齐市园林绿化工程质量监督站(乌鲁木齐市林草种苗站),乌鲁木齐 830092

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羊茅属 芽期 抗旱性 主成分分析 隶属函数法 PEG胁迫

新疆维吾尔自治区重点研发计划新疆维吾尔自治区高等学校基本科研业务费专项

2023B02031-1XJEDU2022J006

2024

种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子:0.502
ISSN:1001-4705
年,卷(期):2024.43(2)
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