Purity and Authenticity Identification of Cucumis melo L.Hybrid Seeds Based on SSR Molecular Markers
This study used the widely planted Cucumis melo L.hybrid'Jinmi6'and the new hybrid variety'Nanjiangmi1'and their parents as experimental materials.Using SSR(Simple repeat sequence molecular marker)detection technology,18 pairs of SSR core primers with rich polymorphism in Cucumis melo L.were selected for purity and authenticity identification.The results showed that'Jin-mi6'and'Nanjiangmi1'obtained multiple pairs of specific primers in 18 pairs of SSR core primer screening,exhibiting polymorphic bands that could be used to identify seed purity.The purity of'Jinmi6'seeds was 97.87％,with specific primers including SSR12833(A),CMBR052(C),ECM147(I),CMBR002(K),CM26(L),MU9175-1(M),MU5554-1(N),SSR00398(O),TJ10(Q),CMBR154(R),of which there were clear bands and strong specificity for primers C,I,K,N,O.The seed purity of'Nanjiangmi 1'was 97.56％,with specific primers such as SR12833(A),CMBR097(F),MU4104-1(G),NR38(H),ECM147(I),CMBR002(K),MU9175-1(M),MU5554-1(N),and CMBR154(R).Primers numbered F,G,H,I,N,and R had clear bands and strong specificity.The results of SSR molecular marker identification were consistent with those of field identification.
万方数据
维普
