摘要
以金线莲组培苗为试验材料,研究添加不同浓度(0、1.5、3、6、9 mmol/L)Ca2+对金线莲组培苗的气孔细胞、农艺性状、酶活性的影响.结果表明,在1.5 mmol/L Ca2+环境下培养的金线莲叶片宽大、植株也相对健壮,总体长势较好,生理生化指标体现该浓度下的金线莲各类代谢活动较为旺盛,各种关键酶的活性也比较高,抗逆性能较优,6 mmol/L Ca2+环境下培养的金线莲叶片较小且幼嫩,但植株丛芽多而健壮.因此,1.5 mmol/L Ca2+是相对适宜金线莲组培苗生长发育的钙环境,而6 mmol/L Ca2+是相对适宜金线莲长芽扩繁的钙环境.
Abstract
In order to investigate the effects of different Ca2+concentrations(0,1.5,3,6,9 mmol/L)on stomatal cells,agronomic characters and enzyme activities of tissue culture seedlings of Anoec-tochilus roxburghii(Wall.)Lindl,tissue culture plantlets of A.chinensis were used as experimental materials.The results showed that,in 1.5 mmol/L Ca2+environment,the leaves of Anoectochilus roxburghii(Wall.)Lindl were broad,the plants are relatively fat,and the overall growth was good.Physiological and biochemical indicators showed that the metabolic activities of Anoectochilus rox-burghii(Wall.)Lindl at this concentration were vigorous,the activities of various key enzymes were relatively high,and the stress resistance was better.Under the condition of 6 mmol/L Ca2+,the leav-es were small and young,but the plants had many and strong plexus buds.Therefore,1.5 mmol/L Ca2+was a relatively suitable calcium environment for the growth and development of tissue culture seedlings,while 6 mmol/L Ca2+was a relatively suitable calcium environment for the growth and propagation of long buds of Anoectochilus roxburghii(Wall.)Lindl.
基金项目
中央引导地方科技发展专项(桂科ZY20198018)
广西中医药局自筹经费科研课题(GXZYA20220003)
广西科技基地和人才专项(桂科AD22080012)
广西壮族自治区中医药重点学科建设项目(GZXK-Z-20-67)
万方数据