首页|2个佛手瓜品种核型分析及基因组大小测定

2个佛手瓜品种核型分析及基因组大小测定

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本试验以贵州省惠水县主要品种绿皮佛手瓜、白皮佛手瓜为材料,通过去壁低渗法染色体制片、流式细胞术分别对其核型、基因组大小,染色体数量、倍性等特征进行研究.结果表明,贵州惠水白皮佛手瓜、绿皮佛手瓜均为2倍体,染色体数目均为28条,白皮佛手瓜、绿皮佛手瓜的核型公式均为2n=2x=28m,白皮佛手瓜核型类型为1B型,绿皮佛手瓜为1C型,绿皮佛手瓜的不对称系数为52.38%,白皮佛手瓜的不对称系数为52.07%,通过流式细胞术测定,白皮佛手瓜、绿皮佛手瓜基因组大小分别为(0.73±0.02)Gb、(0.70±0.02)Gb.
Karyotyping Analysis and Genome Size Determination of Two Sechium edule Varieties
In this study,the karyotype,genome size,chromosome number and ploidy of the main vari-eties of green and white Sechium edule in Huishui County,Guizhou Province were studied by chro-mosome dissection and flow cytometry.The results showed that both white-skinned and green-skinned Sechium edule were diploid and had 28 chromosomes.The karyotype formulas of white-skinned Sechium edule and green-skinned Sechium edule were 2n=2x=28m,the karyotype of white-skinned Sechium edule was type 1B,and that of green-skinned Sechium edule was type 1C.The asymmetry coefficient of green-skinned Sechium edule was 52.38%,and the asymmetry coefficient of white-skinned Sechium edule was 52.07%,by flow cytometry,the genome size of white-skinned and green-skinned Sechium edule was(0.73±0.02)Gb and(0.70±0.02)Gb,respectively.

white-skinned Sechium edulegreen-skinned Sechium edulechromosomeflow cytometry

王维泽、刘炼、赵宁、瞿飞、黎瑞君、文林宏、张朝君、陈之林、李裕荣

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贵州省园艺研究所,贵阳 550006

贵州省农业科技信息研究所,贵阳 550006

白皮佛手瓜 绿皮佛手瓜 染色体 流式细胞术

贵州省科技计划贵州省科技计划——"百"层次人才项目贵州省科技计划贵州省科技计划

黔科合支撑[2021]一般210黔科合平台人才[2016]5671号黔科合服企[2022]005-H-2黔科合支撑[2021]一般211

2024

种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子:0.502
ISSN:1001-4705
年,卷(期):2024.43(3)
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