In order to promote the protection and utilization of the resources of Stephania tetrandra S.Moore,a plant tissue culture system was established using stem segments and callus tissue as materi-als,and suspension culture was carried out using callus tissue cells.Result showed that the optimal culture medium for direct differentiation of adventitious buds from stem segments was MS+6-BA 1.5 mg/L+NAA 0.2 mg/L.The induction rate of adventitious buds was 100％after 60 days,with 16 adventitious buds differentiated from a single explant and up to 28 at 90 days.This formula was also suitable for inducing callus tissue differentiation of adventitious buds,MS+IAA 0.2 mg/L was the best method for adventitious bud rooting,with a rooting rate of 92.5％after 30 days,an average main root length of 7.78 cm,and a survival rate of 88.00％after 15 days of seedling cultivation and trans-plantation.Suspension culture using callus cells could achieve significant bioaccumulation in a shorter culture cycle,and the qualitative detection of alkaloids in dry culture extract was positive.
维普
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