Identification of ear length related genes,revealing their genetic structure variation and excellent haplotypes,which showed of great theoretical and practical significance in the genetic mech-anism dissecting and yield traits improvement by using molecular assistance method.In this study,the ear length related gene RSH2 cloned in previous report,and 120 maize inbred lines derived from tropical and temperate regions were selected as research materials,the genetic structure variation of RSH2 among 120 lines was analyzed by using traditional sequencing technology,and the ear length were evaluated under different environments.Results showed that 28 variant sites,including 24 SNPs and 4 indels,located in RSH2 exons.17 haplotypes were also found,of which Hap_5 showed domi-nant,which was found in 35 lines.The candidate genes association analysis showed that,three muta-tion sites were significantly associated with ear length(p＜0.05),and there was one mutation of G/A at 3 946 bp of RSH2 exon region,resulted to the mutation of TGC encoding Cysteine(C),changed to a L-cysteine encoding Tyrosine(Y)TAC,which could show a significant increase in ear length.Study suggested that site of S_3946 may be a key functional locus for RSH2 gene to control ear length varia-tion,and could be used in maize ear length improvement.
维普
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