温热玉米种质间穗长基因RSH2的遗传结构变异与优异单倍型分析

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中文摘要：鉴定穗长相关基因并揭示其遗传结构变异和优异单倍型,对深度剖析玉米产量形成的遗传机制和基于分子辅助的产量性状改良具有十分重要的理论意义和实践应用价值.本研究在前期图位克隆穗长相关基因RSH2的基础上,以西南地区玉米育种实践中广泛利用的120份温热玉米自交系组成的关联分析群体为材料,利用传统测序技术鉴定RSH2基因在该群体中的遗传结构变异,并结合该群体在不同环境条件下的穗长表型数据,剖析该基因的遗传结构变异和优异单倍型类型.结果表明,在RSH2基因外显子区域中共检测出28个变异位点,包括24个SNPs和4个InDel,共形成17个单倍型,其中Hap_5为优势单倍型,在35份材料中均存在.候选基因关联分析发现,3个突变位点与穗长显著关联(p＜0.05),位于外显子区3 946 bp处存在G/A突变,编码半胱氨酸(Cysteine,C)的密码子TGC突变成编码酪氨酸(Tyrosine,Y)T AC密码子,穗长显著提高.研究表明,位点S_3946可能是RSH2基因控制穗长变异的关键功能位点,可用于后续基于分子辅助的玉米穗长性状改良.

外文标题：The Analysis of Genetic Structure Variation and Major Haplotype of RSH2 Gene among Maize Germplasm Derived from Tropical and Temperate Region

外文摘要：Identification of ear length related genes,revealing their genetic structure variation and excellent haplotypes,which showed of great theoretical and practical significance in the genetic mech-anism dissecting and yield traits improvement by using molecular assistance method.In this study,the ear length related gene RSH2 cloned in previous report,and 120 maize inbred lines derived from tropical and temperate regions were selected as research materials,the genetic structure variation of RSH2 among 120 lines was analyzed by using traditional sequencing technology,and the ear length were evaluated under different environments.Results showed that 28 variant sites,including 24 SNPs and 4 indels,located in RSH2 exons.17 haplotypes were also found,of which Hap_5 showed domi-nant,which was found in 35 lines.The candidate genes association analysis showed that,three muta-tion sites were significantly associated with ear length(p＜0.05),and there was one mutation of G/A at 3 946 bp of RSH2 exon region,resulted to the mutation of TGC encoding Cysteine(C),changed to a L-cysteine encoding Tyrosine(Y)TAC,which could show a significant increase in ear length.Study suggested that site of S_3946 may be a key functional locus for RSH2 gene to control ear length varia-tion,and could be used in maize ear length improvement.

外文关键词：

maizeear lengthgenetic structure variationRSH2excellent haplotype

作者：

涂亮、郭向阳、刘鹏飞、蒋喻林、王安贵、祝云芳、吴迅、陈泽辉

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作者单位：

贵州省农业科学院旱粮研究所,贵阳 550006

农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵阳 550006

关键词：

玉米穗长遗传结构变异 RSH2 优异单倍型

基金：

国家自然科学基金国家自然科学基金国家重点研发计划黔农科青年基金贵州省科技支撑计划贵州省山地农业关键核心技术攻关项目贵州省育种科研基础平台创新能力建设项目贵州省自然科学基金黔农科院国基后补助项目黔农科院国基后补助项目黔农科院国基后补助项目贵州喀斯特山区重要作物生物育种平台建设项目科研机构创新能力建设项目

项目编号：

32060460321604512023YFD1201103202419号黔科合支撑[2022]重点029GZNYGJHX-2023012黔科合服企[2022]014黔科合基础[2022]一般236[2021]16号[2022]02号[2022]09号黔科合中引地[2023]033[2022]007

出版年：

2024

DOI：

10.16590/j.cnki.1001-4705.2024.04.001

种子

贵州省种子管理站贵州省种子学会中国种子协会

种子

CSTPCD北大核心

影响因子：0.502

ISSN：1001-4705

年,卷(期)：2024.43(4)

参考文献量31