Identification and Expression Pattern Analysis of ARR-B Gene Family in Camellia sinensis
As a positive regulator of cytokinin signal transduction,ARR-B gene plays an important role in plant development and stress resistance.This study was based on the Camellia sinensis genome database,using bioinformatics software to successfully identify 23 Camellia sinensis ARR-B genes,and their gene structure,evolutionary relationship,conserved domains,chromosome location,promoter cis-acting clements,and transcriptome data in eight major tissues and four stresses were analysed.Based on the above data,the Fuding Dabai Camellia sinensis was subjected to salt stress treatment and the expression level was verified by fluorescent quantitative PCR technology.The results showed that there were 23 members of the Camellia sinensis ARR-B gene family and they were unevenly distributed on different chromosomes;accord-ing to the phylogenetic tree,the Camellia sinensis ARR-B genes were divided into 6 categories and were homologous to the Arabidopsis thaliana.The gene structure and conserved domains of the same subfamily of the Camellia sinensis ARR-B gene were basically the same;The Camellia sinensis ARR-B gene had higher expression in stem and root tissues and under drought,low temperature,salt and methyl jasmonate stress.There were differential expressions under treatment.Real-time fluorescent quantitative PCR results showed that after salt stress treatment,the expression levels of ARR-B genes in 8 Camellia sinensiss were different from the transcriptome data.This study preliminarily analyzed the functions and anti-stress effects of the ARR-B gene family of Camellia sinensis,and provided reference and basis for further research on the growth and development of Camellia sinensis.
万方数据
维普
