摘要
本研究利用0.5％盐胁迫对275份小麦品种(系)进行芽期耐盐性鉴定,测定发芽率、芽长、根长、根数、芽鲜质量、根鲜质量及根冠比等指标.利用主成分分析法将7个指标转化为3个主成分,累积贡献率为74.35％.通过主成分贡献率和隶属函数分析进一步将3个主成分简化为综合评价值(D).根据D值的大小,利用聚类分析法将275份小麦品种(系)划分为高耐盐、中耐盐、耐盐、盐敏感和盐高敏感共5个等级,筛选出高耐盐种质11份、中耐盐107份、耐盐60份,累计占比64.7％.通过对13个小麦品种(系)进行TaHKT1基因序列分析,发现T∶T新单倍型与耐盐性相关.利用KASP标记对275份小麦品种(系)进行检测,有98份小麦品种(系)为新单倍型,占35.64％;新单倍型小麦品种(系)在各省份中所占比例不同.将小麦品种(系)的TaHKT1基因T∶T单倍型与耐盐性D值进行关联分析,结果表明,TaHKT1基因T:T单倍型与小麦芽期耐盐性差异达极显著水平(p＜0.01).
Abstract
In this study,under the 0.5％salt stress treatment,275 wheat varieties(lines)were identi-fied for salt tolerance during the germination stage,and relative indicators such as germination rate,shoot length,root length,root number,shoot fresh weight,root fresh weight,and root crown ratio were measured.The salt tolerance of 7 indicators was simplified into 3 principal components using principal component analysis,with a cumulative contribution rate of 74.35％.Through the analysis of principal component contribution rate and membership function,the 3 principal component values were further reduced to D-value of comprehensive evaluation index.According to D-value and cluster analysis,275 wheat varieties(lines)were divided into 5 classes of salt resistance,including high salt tolerance,salt tolerance,medium salt tolerance,salt sensitivity,and high salt sensitivity.Eleven,one hundred and seven,and sixty wheat varieties(lines)with high salt tolerance,medium salt toler-ance,and salt tolerance above were selected,accounting for 64.7％of the total.The TaHKT1 gene sequence analysis of 13 wheat varieties(lines)revealed that the new T∶T haplotype was related to salt tolerance.275 wheat varieties(lines)were tested by KASP markers,98 of which were the new haplo-type,accounting for 35.64％.The proportion of new haplotype wheat varieties(lines)in different provinces were different.The correlation analysis between the T∶T genotype of wheat varieties(lines)and the salt tolerance D-value showed that the new haplotype of TaHKT1 gene was significantly dif-ferent from the salt tolerance of wheat at the bud stage(p＜0.01).
基金项目
河北省耐盐碱作物种业创新团队项目(23327501D)
河北省重点研发计划(现代种业科技创新专项)(21326318D)
财政部和农业农村部现代农业产业技术体系建设专项(CARS-03)
维普
万方数据