In order to solve the problem of low success rate of natural crossing and low breeding efficiency of hybrid seedlings,the cross breeding system of Pinellia ternata(Thunb.)Breit was constructed and the prop-agation cycle of hybrid seedlings was studied.TTC method was used to determine the vitality of the sterilized Pinellia ternata(Thunb.)Breit pollen and pistil,and the best medium for pistil was determined through comparative experiment.F1 generation hybrid seedlings were continuously cultivated for three new Pinellia ternata(Thunb.)Breit strains,and the transition cycle and seedling formation cycle were explored through the hybrid seedling subgeneration.The results showed that the optimum disinfection time for pistil and pollen of Pinellia ternata(Thunb.)Breit was 6 min.The optimal medium formulation for pistil was semi-saccha-rose AGAR free MS medium 20.00 g/L+sucrose 15.00 g/L+AGAR 5.00 g/L+6-BA 1.00 mg/L+NAA 0.01 mg/L,pH=5.8.On this basis,F1 substitute seedlings were cultivated.There was obvious difference in leaf type between F1 hybrid seedlings of 4 Pinellia ternata(Thunb.)Breit strains and their parent tissue cul-ture seedlings,and the propagation cycle of two hybrid seedlings was found after propagation.In this study,a Pinellia ternata(Thunb.)Breit hybrid system based on plant tissue culture technology was established and the hybrid seedlings were obtained,and the breeding cycle of the hybrid seedlings was proved.
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