刺梨果实不同发育时期转录组分析及RACE法克隆CNR基因
Transcriptome Analysis and Cloning of CNR Gene by RACE Method of Rosa roxburghii Tratt.Fruit at Different Developmental Stages
陈宏宇 1杨烨 1于莹 2田媛1
作者信息
- 1. 贵州中医药大学药学院,贵阳 550025
- 2. 贵州中医药大学基础医学院,贵阳 550025
- 折叠
摘要
为研究刺梨果实不同发育期的差异表达基因,对不同发育期果实进行转录组测序.结果表明,共组装了 98 752个单基因,其中有64003个基因可被数据库注释.Ⅰ期vsⅡ期有上调基因1 842个,下调基因1 159个;Ⅰ期vs Ⅲ期有上调基因911个,下调基因538个.刺梨果实不同时期的基因表达差异较大,这些基因表达差异为探索刺梨果实发育机制提供参考.CNR基因即细胞数量调控因子,是一个植物的细胞数量负调控因子,可能调控果实发育.本研究利用RACE技术克隆得到一个刺梨CNR基因的cDNA全长序列,并构建真核表达载体,为后续深入研究基因功能提供基础.
Abstract
In order to study the differentially expressed genes of Rosa roxburghii Tratt.fruits at differ-ent development stages,transcriptome sequencing was performed on the fruits at different develop-ment stages.The results showed that a total of 98 752 single genes were assembled,of which 64 003 genes could be annotated by the database.There were 1 842 up-regulated genes and 1 159 down-regu-lated genes in stage Ⅰ vs stage Ⅱ.There were 911 up-regulated genes and 538 down-regulated genes in stage Ⅰ vs stage Ⅲ.The differences in gene expression in different periods of Rosa roxburghii Tratt.fruit were big,which provided a reference for exploring the mechanism of Rosa roxburghii Tratt.fruit development.CNR gene,cell number regulator,was a negative regulator of plant cell number and could regulate fruit development.In this study,a full-length cDNA sequence of CNR gene was cloned using RACE technology,and the eukaryotic expression vector was constructed,which provided the basis for further study of gene function.
关键词
刺梨/果实/转录组/细胞数量调控因子/基因Key words
Rosa roxburghii Tratt./fruit/transcriptome/cell number regulator factors/gene引用本文复制引用
基金项目
国家自然科学基金(82360853)
贵州中医药大学学术新苗项目(贵科合学术新苗[2023]-18号)
贵州中医药大学博士启动基金([2020]19号)
出版年
2024
国家科技期刊平台
NSTL
万方数据