Transcriptome Analysis and Cloning of CNR Gene by RACE Method of Rosa roxburghii Tratt.Fruit at Different Developmental Stages
In order to study the differentially expressed genes of Rosa roxburghii Tratt.fruits at differ-ent development stages,transcriptome sequencing was performed on the fruits at different develop-ment stages.The results showed that a total of 98 752 single genes were assembled,of which 64 003 genes could be annotated by the database.There were 1 842 up-regulated genes and 1 159 down-regu-lated genes in stage Ⅰ vs stage Ⅱ.There were 911 up-regulated genes and 538 down-regulated genes in stage Ⅰ vs stage Ⅲ.The differences in gene expression in different periods of Rosa roxburghii Tratt.fruit were big,which provided a reference for exploring the mechanism of Rosa roxburghii Tratt.fruit development.CNR gene,cell number regulator,was a negative regulator of plant cell number and could regulate fruit development.In this study,a full-length cDNA sequence of CNR gene was cloned using RACE technology,and the eukaryotic expression vector was constructed,which provided the basis for further study of gene function.
Rosa roxburghii Tratt.fruittranscriptomecell number regulator factorsgene
NETL
NSTL
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