为探究大蕉果实不同发育时期在转录水平上的变化及类胡萝卜素生物合成代谢的差异表达基因,本研究以幼果期(BGZ1)、成熟期(BGZ2)、后熟期(BGZ3)的大蕉果实为研究对象,借助转录组测序技术进行分析.差异表达基因分析结果显示,BGZ1 vs BGZ2、BGZ1 vs BGZ3和BGZ2 vs BGZ3差异表达基因数目分别为3 828个、16 138个和14 923个.GO分类和富集分析显示,3个差异分组主要富集在代谢过程、发育过程、结合、催化活性、细胞解剖实体等.KEGG分析结果表明,3个差异分组中,与类胡萝卜素的合成代谢相关的代谢途径、次生代谢产物的生物合成途径富集比较多.BGZ1 vs BGZ2、BGZ2 vs BGZ3分别有556个、1 507个显著差异表达的转录因子,BGZ1 vs BGZ2显著差异表达的转录因子中下调表达居多.参与大蕉果实类胡萝卜素合成代谢的差异候选基因有37个,与BGZ1相比,BGZ2有12个基因上调表达、27个基因下调表达;与BGZ2相比,BGZ3有17个基因上调表达、20个基因下调表达.3个PSY候选基因的表达量在BGZ3时期降低;LCYE和LCYB在后熟期具有较高基因表达量.研究结果为大蕉中类胡萝卜素代谢的分子调控机制提供参考.
Transcriptomic Analysis of Musa sapientum Fruit and Study on Genes Involved in Carotenoid Biosynthesis
In order to explore the transcriptional changes of Musa sapientum fruits at different develop-mental stages and the differentially expressed genes of carotenoid bioanabolism,this study took Musa sapientum fruits at young fruit stage,mature stage and post-ripe stage as research objects,and ana-lyzed them by transcriptome sequencing technology.The results of gene analysis showed that the number of differentially expressed genes of BGZ1 vs BGZ2,BGZ1 vs BGZ3 and BGZ2 vs BGZ3 were 3 828,16 138 and 14 923,respectively.GO classification and enrichment analysis showed that the three differential groups were mainly concentrated in metabolic process,developmental process,bind-ing,catalytic activity,and cell anatomical entity.KEGG analysis showed that among the three differ-ent groups,more metabolic pathways related to carotenoid anabolism and biosynthesis pathways of secondary metabolites were enriched.BGZ1 vs BGZ2,BGZ2 vs BGZ3 had 556 and 1 507 significantly differentially expressed transcription factors,respectively,and BGZ1 vs BGZ2 significantly differenti-ally expressed transcription factors were mostly down-regulated.There were 37 different candidate genes involved in carotenoid anabolism in Musa sapientum.Compared with BGZ1,12 BGZ2 genes were up-regulated and 27 genes were down-regulated.Compared with BGZ2,BGZ3 had 17 up-regula-ted genes and 20 down-regulated genes.The expression levels of three PSY candidate genes decreased during BGZ3 period.LCYE and LCYB had higher gene expression levels in late maturation.The re-sults provided a reference for the molecular regulation mechanism of carotenoid metabolism in Musa sapientum.
Musa sapientumtranscriptomecarotenoidtranscription factor
NETL
NSTL
万方数据
