黄花苜蓿MfMYB3R-3基因克隆及结构与功能的生物信息学分析
Cloning of MfMYB3R-3 Gene of Medicago falcata L.and Bioinformatics Analysis of Structure and Function
柴华 1杨瞾 2申忠宝 3李莎莎 2王晓龙 2徐艳霞 2吴玥2
作者信息
- 1. 黑龙江省农业科学院博士后科研工作站,哈尔滨 150086;黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔 161005
- 2. 黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔 161005
- 3. 黑龙江省农业科学院草业研究所,哈尔滨 150086
- 折叠
摘要
MYB转录因子是植物中分布最广的转录因子家族之一,在植物响应生物及非生物胁迫,调控逆境胁迫相关基因表达方面起着重要的作用.本研究基于黄花苜蓿转录组数据,鉴定和克隆了黄花苜蓿MfMYB3R-3基因,并进行了生物信息学分析.结果表明,MfMYB3R-3基因CDS区长1 701 bp,编码566个氨基酸,具有SANT保守结构域,为MYB3R型转录因子;MfMYB3R-3蛋白化学分子式为C2690 H4314 N796 O873 S20,理论等电点为8.75,该蛋白是亲水性不稳定偏碱性非分泌蛋白,不具有信号肽,无跨膜结构,亚细胞定位预测在细胞核.系统进化分析发现,黄花苜蓿MfMYB3R-3蛋白与蒺藜苜蓿的MYB3R-3的亲缘关系最近.
Abstract
MYB transcription factor is one of the most widely distributed transcription factor families in plants,and plays an important role in regulating the expression of stress-related genes in response to biological and abiotic stresses.In this study,based on the transcriptome data of Medicago falcata L.,MfMYB3R-3 gene was identified and cloned,and the bioinformatics was analyzed.The results showed that coding sequence of MfMYB3R-3 was 1 701 bp long,with 566 amino acids encoded and SANT preserving structure domain,which was the MYB3R type transcription factor.MfMYB3R-3 protein chemical formula was C2690 H4314 N796 O873 S20,theoretical isoelectric point was 8.75,this protein was hydrophilic unstable alkaline non-secretory protein,no signal peptide,no transmembrane struc-ture,subcellular localization predicted in the nucleus.The phylogenetic analysis showed that MfMYB3R-3 protein of Medicago falcata L.was closely related to MYB3R-3 of Medicago truncatula.
关键词
黄花苜蓿/MfMYB3R-3基因/克隆/生物信息学Key words
Medicago falcata L./MfMYB3R-3 gene/clone/bioinformatics引用本文复制引用
基金项目
黑龙江省博士后面上项目(LBH-Z22259)
黑龙江省农科院农业科技基础创新项目(CX23YQ10)
出版年
2024
国家科技期刊平台
NSTL
万方数据