MYB transcription factor is one of the most widely distributed transcription factor families in plants,and plays an important role in regulating the expression of stress-related genes in response to biological and abiotic stresses.In this study,based on the transcriptome data of Medicago falcata L.,MfMYB3R-3 gene was identified and cloned,and the bioinformatics was analyzed.The results showed that coding sequence of MfMYB3R-3 was 1 701 bp long,with 566 amino acids encoded and SANT preserving structure domain,which was the MYB3R type transcription factor.MfMYB3R-3 protein chemical formula was C2690 H4314 N796 O873 S20,theoretical isoelectric point was 8.75,this protein was hydrophilic unstable alkaline non-secretory protein,no signal peptide,no transmembrane struc-ture,subcellular localization predicted in the nucleus.The phylogenetic analysis showed that MfMYB3R-3 protein of Medicago falcata L.was closely related to MYB3R-3 of Medicago truncatula.
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