Cloning of MfMYB3R-3 Gene of Medicago falcata L.and Bioinformatics Analysis of Structure and Function
MYB transcription factor is one of the most widely distributed transcription factor families in plants,and plays an important role in regulating the expression of stress-related genes in response to biological and abiotic stresses.In this study,based on the transcriptome data of Medicago falcata L.,MfMYB3R-3 gene was identified and cloned,and the bioinformatics was analyzed.The results showed that coding sequence of MfMYB3R-3 was 1 701 bp long,with 566 amino acids encoded and SANT preserving structure domain,which was the MYB3R type transcription factor.MfMYB3R-3 protein chemical formula was C2690 H4314 N796 O873 S20,theoretical isoelectric point was 8.75,this protein was hydrophilic unstable alkaline non-secretory protein,no signal peptide,no transmembrane struc-ture,subcellular localization predicted in the nucleus.The phylogenetic analysis showed that MfMYB3R-3 protein of Medicago falcata L.was closely related to MYB3R-3 of Medicago truncatula.
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