Genetic Diversity and Population Structure Analysis of the Perilla frutescens L.Germplasm Resources Based on SSR Markers
In order to clarify the genetic basis of different Perilla frutescens L.germplasm and popula-tion relationship,19 pairs of SSR molecular markers were used to analyze the genetic diversity and population structure of 141 Perilla frutescens L.resources from different geographical sources.The results showed that 80 polymorphic bands were amplified from 19 pairs of SSR markers,with an av-erage of 4.26 bands per primer pair.The polymorphism index(PIC)ranged from 0.331 1 to 0.845 7,with an average of 0.620 6 bands,accounting for 85.10％.Molecular clustering of 141 Perilla was per-formed by UPGMA method,and the genetic similarity coefficient(GS)ranged from 0.478 7 to 0.948 9,with an average of 0.709 9.The materials were divided into three classes at GS=0.696.Through the analysis of Structure and population structure,the tested materials were divided into 3 groups.Among them,68.8％of Perilla frutescens L.germplasm resources had a single genetic back-ground,31.2％had mixed ancestry,and their genetic background was more complex.
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