外源甜菜碱对高温胁迫下生菜种子萌发的影响
Effect of Exogenous Betaine on Germination of Lactua sativa L.Seeds Under High Temperature
杨志杰 1陈艳 2戴陶宇 1宋云鹏 1张迎迎1
作者信息
- 1. 上海市农业科学院园艺研究所/上海市设施园艺技术重点实验室,上海 201403
- 2. 上海市农业科学院园艺研究所/上海市设施园艺技术重点实验室,上海 201403;上海海洋大学水产与生命学院,上海 201306
- 折叠
摘要
本研究以生菜高温敏感品种"黑木耳"为试验材料,研究外源甜菜碱对高温胁迫下生菜种子萌发的影响.结果表明,高温胁迫下,经过甜菜碱预处理后的生菜种子发芽率、发芽指数和种子活力与对照相比均显著提高.对种子相关生理指标进行测定,发现丙二醛(MDA)、过氧化氢(H2O2)和超氧阴离子(O2·-)与对照相比含量显著下调,而脯氨酸(Pro)含量增加,同时过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)三种酶活性显著上调.说明高温胁迫导致生菜种子中活性氧(ROS)水平升高并引起过氧化反应从而抑制种子萌发,外源甜菜碱可以通过增强抗氧化系统、清除活性氧,以及累积Pro,进而提高生菜种子的耐高温能力,起到很好的种子引发作用.
Abstract
The effect of exogenous betaine on the germination of Lactua sativa L.seeds under high temperature stress was studied by using the high temperature sensitive variety'black fungus'as experi-mental material.The results showed that the germination rate,germination index and seed vigor of Lactua sativa L.seeds treated with betaine were significantly increased under high temperature stress compared with the control.Compared with the control,the content of malondialdehyde(MDA),hydrogen peroxide(H2O2)and superoxide anion(O2·-)significantly decreased,while the content of proline(Pro)increased.Meanwhile the activities of catalase(CAT),peroxidase(POD)and superox-ide dismutase(SOD)were significantly up-regulated.These results indicated that high temperature stress led to the increase of ROS levels in Lactua sativa L.seeds and caused peroxidation reaction,thus inhibiting seed germination.Exogenous betaine could enhance the high temperature resistance of Lactua sativa L.seeds by enhancing the antioxidant system,removing ROS and accumulating Pro,thus playing a good role in seed initiation.
关键词
生菜种子/萌发/甜菜碱/高温胁迫/耐高温Key words
Lactua sativa L.seed/germination/betaine/high temperature stress/high temperature tolerance引用本文复制引用
基金项目
上海市崇明区农业科技创新项目(沪农科产字[2023]第2号)
出版年
2024
国家科技期刊平台
NSTL
万方数据