Analysis of the Composition and Content of Storage Protein Subunits in the Soybean Qiandou Series of Varieties
In order to determine the contents of soybean globulin and its subunit components in Qian-dou series soybean varieties(strains),so as to promote the breeding and application of new soybean varieties specially for nutrition or processing.The composition of 7S and 11S globulin subunits,rela-tive percentage and 11S/7S ratio of 41 soybean varieties(lines)were analyzed by polyacrylamide gel electrophoresis(SDS-PAGE).The results showed that there were significant differences in the rela-tive percentage of soybean protein and its subunit components among different varieties.The average relative percentage content of 7S and 11S globulin in 41 tested varieties(lines)was 26.93%and 54.92%,the variation ranges were from 15.58%to 43.89%,37.10%to 66.48%,and the coefficient of variation was 29.36%and 13.57%,respectively.The 11S/7S values ranged from 0.85 to 4.05,the mean value was 2.28,and the coefficient of variation was 38.71%.The coefficient of variation of 7S protein was higher than that of 11S protein,the coefficient of variation of β subunit of 7S protein was the largest,and the coefficient of variation of B subunit of 11S protein was the largest.11 varieties with 11S/7S value higher than 3 were screened,and 2 varieties with 11S/7s value higher than 4 were screened.There was a significant negative correlation between 7S and 11S globulin components.The 41 varieties(strains)were grouped into 3 groups.The average content of 11S protein and 11S/7S in Group Ⅰ was the highest,the average content of A4 and A1 A2 subunits in Group Ⅱ was the highest,and the average content of 7S protein and A3 subunits in Group Ⅲ was the highest.
Qiandou soybeanstorage proteincomposition of subunits