In order to determine the contents of soybean globulin and its subunit components in Qian-dou series soybean varieties(strains),so as to promote the breeding and application of new soybean varieties specially for nutrition or processing.The composition of 7S and 11S globulin subunits,rela-tive percentage and 11S/7S ratio of 41 soybean varieties(lines)were analyzed by polyacrylamide gel electrophoresis(SDS-PAGE).The results showed that there were significant differences in the rela-tive percentage of soybean protein and its subunit components among different varieties.The average relative percentage content of 7S and 11S globulin in 41 tested varieties(lines)was 26.93%and 54.92%,the variation ranges were from 15.58%to 43.89%,37.10%to 66.48%,and the coefficient of variation was 29.36%and 13.57%,respectively.The 11S/7S values ranged from 0.85 to 4.05,the mean value was 2.28,and the coefficient of variation was 38.71%.The coefficient of variation of 7S protein was higher than that of 11S protein,the coefficient of variation of β subunit of 7S protein was the largest,and the coefficient of variation of B subunit of 11S protein was the largest.11 varieties with 11S/7S value higher than 3 were screened,and 2 varieties with 11S/7s value higher than 4 were screened.There was a significant negative correlation between 7S and 11S globulin components.The 41 varieties(strains)were grouped into 3 groups.The average content of 11S protein and 11S/7S in Group Ⅰ was the highest,the average content of A4 and A1 A2 subunits in Group Ⅱ was the highest,and the average content of 7S protein and A3 subunits in Group Ⅲ was the highest.
Qiandou soybeanstorage proteincomposition of subunits