基于ISSR和TRAP分子标记的东北地区栽培黑木耳遗传多样性研究
Genetic Diversity Analysis of Cultivated Auricularia heimuer Based on ISSR and TRAP Molecular Markers
焦枥禾 1张丕奇 1王凤利 1张秀丰 2岳欣 1戴肖东1
作者信息
- 1. 黑龙江省科学院微生物研究所,哈尔滨 150010
- 2. 黑龙江华腾生物科技有限公司,黑龙江佳木斯 154000
- 折叠
摘要
为探究东北地区黑木耳栽培菌株的遗传多样性及亲缘关系,采用ISSR和TRAP分子标记对50株栽培黑木耳菌株进行遗传多样性分析.利用22条高多态性、高分辨率的ISSR和TRAP分子标记引物,对栽培黑木耳进行了 DNA扩增.结果表明,黑木耳ISSR和TRAP标记谱带多态性较高,分别占89.58％和84.71％,遗传相似系数为0.53～1.00;在ISSR标记的聚类图中相似系数大于0.96的菌株占44.0％,TRAP标记的聚类图中相似系数大于0.96的菌株占54.0％;STRUCTURE聚类结果显示,50株菌株可分为四大类;遗传多样性分析结果表明,东北地区黑木耳栽培菌株的遗传多样性主要来源于群体间,黑木耳栽培菌株的遗传多样性欠丰富,生产菌种资源的同质化较为突出.
Abstract
In order to explore the genetic diversity and genetic relationship of cultivated Auricularia heimuer strains in northeast China,genetic diversity of 50 cultivated Auricularia heimuer strains was analyzed by ISSR and TRAP molecular markers.The DNA of cultivated Auricularia heimuer was amplified by 22 highly polymorphic and high-resolution ISSR and TRAP labeled primers.The results showed that the polymorphisms of ISSR and TRAP markers were high,accounting for 89.58％and 84.71％,respectively,with genetic similarity coefficients ranging from 0.53 to 1.00.The strains with similarity coefficient greater than 0.96 accounted for 44.0％in ISSR labeled cluster plots,and 54.0％in TRAP labeled cluster plots.The STRUCTURE clustering results showed that the 50 strains could be divided into four categories.The results of genetic diversity analysis showed that the genetic diver-sity of Auricularia heimuer cultivated strains in northeast China was mainly from inter-population,and the genetic diversity of Auricularia heimuer cultivated strains was not rich,and the homogeneity of production strain resources was prominent.
关键词
黑木耳/遗传多样性/分子标记/ISSR/TRAPKey words
Auricularia heimuer/genetic diversity/molecular markers/ISSR/TRAP引用本文复制引用
基金项目
黑龙江省"揭榜挂帅"科技攻关计划(2022ZXJ06B04)
出版年
2024
国家科技期刊平台
NSTL
万方数据