Construction of Fingerprint and Purity Identification of Main Cultivars of Spring Brassica napus in Qinghai Province
A total of 16 SSR markers with polymorphism,clear bands and good repeatability were selected from 190 pairs of Brassica napus SSR primers.16 pairs of core primers were used to con-struct fingerprints of 15 parent materials of spring Brassica napus varieties.The results showed that a total of 44 loci were amplified by 16 markers,with an average amplification of 2.5 loci per primer pair.The 15 materials were classified into three classes by UPGMA at a genetic distance of 0.65.Two pairs of SSR markers(A04-10,A06-10)were used,and the markers were closely linked to the Polymaa cytoplasmic male sterility restoration gene(Rfp)YSSR3 was used to identify the purity of the hybrids of Qingza No.4,Qingza No.7,Qingza-limited No.1,Qingza No.5,Qingza No.9,Qingza No.15,Qingza No.12,Qingza No.16 and Qingza No.18,which showed that SSR markers were more advantageous for the purity identification of hybrids.
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