摘要
在190对甘蓝型油菜SSR引物中筛选出具有多态性、条带清晰、重复性好的16对SSR标记,使用16对核心引物对青杂系列春油菜品种的15份亲本材料进行指纹图谱构建.结果表明,16个标记共扩增出44个位点,每对引物平均扩增的位点为2.5个,使用UPGMA法将15份材料在遗传距离为0.65处分为三类.同时利用其中的两对SSR标记(A04-10、A06-10)、与波里马细胞质雄性不育恢复基因(Rfp)紧密连锁的标记YSSR3对青杂4号、青杂7号、青杂有限1号、青杂5号、青杂9号、青杂15号、青杂12号、青杂16号、青杂18号的杂交种进行纯度鉴定,表明SSR标记用于杂交种的纯度鉴定更有优势.
Abstract
A total of 16 SSR markers with polymorphism,clear bands and good repeatability were selected from 190 pairs of Brassica napus SSR primers.16 pairs of core primers were used to con-struct fingerprints of 15 parent materials of spring Brassica napus varieties.The results showed that a total of 44 loci were amplified by 16 markers,with an average amplification of 2.5 loci per primer pair.The 15 materials were classified into three classes by UPGMA at a genetic distance of 0.65.Two pairs of SSR markers(A04-10,A06-10)were used,and the markers were closely linked to the Polymaa cytoplasmic male sterility restoration gene(Rfp)YSSR3 was used to identify the purity of the hybrids of Qingza No.4,Qingza No.7,Qingza-limited No.1,Qingza No.5,Qingza No.9,Qingza No.15,Qingza No.12,Qingza No.16 and Qingza No.18,which showed that SSR markers were more advantageous for the purity identification of hybrids.
基金项目
青海省帅才科学家负责制项目(2022-NK-170)
国家科技期刊平台
NSTL
万方数据