首页|基于不同含水量基质下黔藿1号种子萌发过程中代谢组学差异分析

基于不同含水量基质下黔藿1号种子萌发过程中代谢组学差异分析

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
为研究水分对黔藿1号种子萌发的影响,检测育苗基质不同含水量下种子不同萌发阶段相关代谢产物和代谢途径的变化,以黔藿1号种子为试验材料,采用广靶代谢组学方法对育苗基质不同含水量下黔藿1号种子萌发过程的代谢途径及其代谢物变化等进行分析.结果表明,基质含水量为200％时,有利于黔藿1号种子萌发.在基质含水量分别为100％和200％条件下,种子萌发过程中的代谢物种类变化相似,主要有氨基酸及其衍生物、糖类、黄酮、生物碱等,其中差异代谢物显著富集到精氨酸和脯氨酸代谢、鞘磷脂代谢、黄酮类生物合成等通路.在基质含水量200％较100％下,二氢茉莉酸、桂酸桂酯的相对含量随着种子萌发时间后移呈先升后降的趋势,可能是促进种子萌发的原因之一.
Analysis on Metabolomics Differences of Qianhuo No.1 During Seed Germination Based on Different Water Content Substrates
The effects of water on seed germination of Qianhuo No.1(Epimedium sagittatum)were studied,and the changes of related metabolites and metabolic pathways of seeds at different germina-tion stages under different water content in seedling medium were detected.The metabolic pathways and metabolites of Qianhuo No.1 seeds during germination under different water content in seedling medium were analyzed by using broad-target metabolomics method.The results showed that when the water content of medium was 200％,it was favorable for the seed germination of Qianhuo No.1.When the water content of the substrate was 100％and 200％,the metabolites during seed germina-tion had similar changes,mainly including amino acids and their derivatives,sugars,flavonoids,alkaloids,etc.The different metabolites were significantly enriched into arginine and proline metabolism,sphingomyelin metabolism,flavonoid biosynthesis and other pathways.Under the water content of 200％and 100％,the relative contents of dihydrojasmonic acid and cinnamyl cinnamate increased first and then decreased with the time of seed germination,which could be one of the reasons for promoting seed germination.

Qianhuo No.1seed germinationwater contentmetabolomics

熊毅、汪卓逸、汪永平、龙海成、丁邦勇、王华磊、罗春丽、刘红昌

展开 >

贵州大学农学院,贵阳 550025

多伦多大学生命科学学院,加拿大多伦多M5S1A1

贵州贵基生物医药有限公司,贵州从江 557400

贵州省药用植物繁育与种植重点实验室,贵阳 550025

展开 >

黔藿1号 种子萌发 含水量 代谢组学

贵州省科技厅科技成果一般转化项目贵州特色药用植物种苗繁育工程技术研究中心国家重点研发计划项目贵州省药用植物繁育与种植人才基地贵州省粮油作物分子育种重点实验室项目贵州省高等学校功能农业重点实验室项目

黔科合成果[2023]一般030黔科合平台人才[2020]2101号2021YFD1601002黔人领发[2020]2号黔科合中引地[2023]008黔教技[2023]007号

2024

10.16590/j.cnki.1001-4705.2024.06.028

种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子：0.502
ISSN：1001-4705
年,卷(期)：2024.43(6)
  • 12