Cloning and Bioinformatics Analysis of ZmASA2 Gene in Maize
Anthranilate synthase(AS)is a complex enzyme that catalyzes the synthesis of tryptophan in plant cells,consisting of α subunits and β subunits.In this study,a cDNA fragment encodingα subunit homologous gene of anthranilic acid synthase was cloned from corn B73 by RT-PCR tech-nique,named ZmASA2.Amino acid sequence analysis showed that the total length of the cDNA frag-ment was 1 731 bp,and it was predicted to code a polypeptide containing 577 amino acids,with a molecular weight of about 64.48 kD and a theoretical isoelectric point of 6.64.Bioinformatics analysis showed that ZmASA2 was a hydrophilic protein located in the cytoplasm,and its secondary structure was mainly α helix.Phylogenetic analysis showed that ZmASA2 protein was the closest phylogenetic relation to AS of Miscanthus lutarioriparius and sorghum.The results of qRT-PCR showed that the expression of ZmASA2 was the highest at 30 days after pollination,followed by the stem.
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