玉米ZmASA2基因的克隆与生物信息学分析
Cloning and Bioinformatics Analysis of ZmASA2 Gene in Maize
吴培珍 1邓磊 2袁艳丽 1马周杰 1张开武 3任洪 2王伟2
作者信息
- 1. 贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与种质创新教育部重点实验室,贵阳 550025
- 2. 贵州省农业科学院旱粮研究所,贵阳 550006;农业农村部喀斯特山区玉米生物学与遗传育种重点实验室,贵阳 550006
- 3. 贵州省农业科学院旱粮研究所,贵阳 550006
- 折叠
摘要
邻氨基苯甲酸合成酶(Anthranilate synthase,AS),是存在于植物细胞中催化色氨酸合成的复合酶,由α亚基和β亚基组成.本研究采用RT-PCR技术,从玉米B73中克隆得到一个编码邻氨基苯甲酸合成酶α亚基同源基因的cDNA片段,命名为ZmASA2.氨基酸序列分析表明,该cDNA片段全长1 731 bp,预测编码一条含577个氨基酸的多肽,分子量为64.48 kD,理论等电点为6.64.生物信息学分析表明,ZmASA2属于定位在细胞质中的亲水性蛋白,二级结构主要为α螺旋.系统发育分析表明,ZmASA2蛋白与南荻和高粱的AS在进化上种属关系最近.qRT-PCR结果显示,ZmASA2在授粉后30 d籽粒表达量最高,其次是茎.
Abstract
Anthranilate synthase(AS)is a complex enzyme that catalyzes the synthesis of tryptophan in plant cells,consisting of α subunits and β subunits.In this study,a cDNA fragment encodingα subunit homologous gene of anthranilic acid synthase was cloned from corn B73 by RT-PCR tech-nique,named ZmASA2.Amino acid sequence analysis showed that the total length of the cDNA frag-ment was 1 731 bp,and it was predicted to code a polypeptide containing 577 amino acids,with a molecular weight of about 64.48 kD and a theoretical isoelectric point of 6.64.Bioinformatics analysis showed that ZmASA2 was a hydrophilic protein located in the cytoplasm,and its secondary structure was mainly α helix.Phylogenetic analysis showed that ZmASA2 protein was the closest phylogenetic relation to AS of Miscanthus lutarioriparius and sorghum.The results of qRT-PCR showed that the expression of ZmASA2 was the highest at 30 days after pollination,followed by the stem.
关键词
玉米/邻氨基苯甲酸合成酶/生物信息学分析/表达分析Key words
maize/anthranilate synthase/bioinformatics analysis/expression analysis引用本文复制引用
基金项目
国家自然科学基金(32060461)
国家玉米产业技术体系(CARS-02-91)
贵州省科技计划项目(黔科中引地[2022]4011)
贵州省科技计划项目(黔科合服企[2022]007)
出版年
2024
国家科技期刊平台
NSTL
万方数据