摘要
为了揭示不同温度条件下苗期形态性状和耐热性的分子遗传基础,本研究以籼稻中9B和粳稻M494杂交衍生的重组自交系(Recombinant inbred line,RIL)群体为试验材料,结合高密度SNP图谱,在两种温度条件下对水稻苗期株高、根长、地上干重、地下干重、地上鲜重、地下鲜重及高温处理下干枯叶长等级进行QTL分析.结果表明,在两种温度处理下,RIL群体苗期耐热性存在明显差异;共检测到28个苗期形态性状和耐热性相关QTL,分布于第1、2、3、4、7、8、10号染色体上,可解释的表型贡献率介于3.26％～34.10％之间.其中在第3号染色体Chunk343～351区间存在同时控制两种温度条件下株高、地上鲜重、地下鲜重、地上干重、地下干重和高温下干枯叶长等级的主效QTL簇;在第3号染色体Chunk338～343区间存在控制高温条件下根长的QTL;在第8号染色体Chunk796～803区间检测到同时调控高温条件下地上鲜重和地下鲜重的新QTL位点.
Abstract
In order to reveal the seedling morphological characters under different temperature condi-tions and heat resistance of the molecular genetic basis,this study with 9B in indica and japonica M494 recombinant inbred lines derived from hybridization(Recombinant inbred line,RIL)group as the experimental material,combined with high density SNP map,QTL analysis was performed on plant height,root length,above ground dry weight,underground dry weight,above ground fresh weight,underground fresh weight and dry and dead leaf length of Oryza sativa L.after high tempera-ture treatment under two temperature conditions.The results showed that there were obvious differ-ences in heat resistance of RIL population at the seedling stage under two temperature conditions.A total of 28 QTLs related to morphological traits and heat resistance at seedling stage were detected,which were distributed on the 1 st,2 nd,3 rd,4 th,7 th,8 th and 10 th chromatics,and the explica-ble phenotypic tribute rates ranged from 3.26％to 34.10％.Among them,in the Chunk343-351 interval of No.3 dye body,there were major QTL clusters that controlled dry and dead leaf length,plant height,above ground fresh weight,underground fresh weight,above ground dry weight and underground dry weight at the same time under two temperature conditions.There was a QTL in Chunk338-343 of chromosome 3 that controlled root length under high temperature conditions.A new QTL locus was detected in the Chunk796-803 region of chromosome 8,which regulated both above-ground fresh weight and subsurface fresh weight under high temperature conditions.
基金项目
浙江省重点研发项目(2021C02056)
水稻生物育种全国重点实验室自主课题(2023ZZKT20304)
国家科技期刊平台
NSTL
万方数据