基于全基因组关联分析挖掘甘蓝型油菜油酸含量QTL与SNP分子标记
Exploration on QTL and SNP Molecular Markers for Oleic Acid Content in Brassica napus Based on Genome-wide Association Analysis
李敏 1方正 2陈俊锟 3梁龙兵 3向阳2
作者信息
- 1. 贵阳市白云区农业农村局,贵阳 550014
- 2. 贵州省农业科学院/贵州省油菜研究所,农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵阳 550025;贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与种质创新教育部重点实验室,贵阳 550025
- 3. 贵州省农业科学院/贵州省油菜研究所,农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵阳 550025
- 折叠
摘要
本研究以国内外收集的300份甘蓝型油菜种质为材料,在2个环境下种植,结合油酸含量的表型和基因型数据,利用EMMAX软件进行全基因组关联分析.结果表明,关联群体的油酸含量分布呈连续性双峰分布,受2个主效基因位点控制;GWAS分析检测到一个控制油酸含量性状的主效QTL位点,位于C0 3染色体的第53853975～57808820位碱基之间,贡献率为62.51％,与chrC03_53853975(A/G)、chrC03_57808820(C/T)SNP分子标记及峰值SNP分子标记chrC03_56392730(G/T)紧密连锁,对应碱基突变导致多态性.峰值SNP等位基因为TT时,该性状表型效应值为57.93％.本研究首次揭示一种甘蓝型油菜籽油酸含量性状的C03染色体主效QTL位点及其SNP分子标记,贡献率高,为该性状的高效改良与分子标记辅助选择提供了重要理论依据.
Abstract
In this study,300 Brassica napus germplasms collected from both domestic and internation-al sources were planted in two environments.By integrating phenotypic and genotypic data of oleic acid content,genome-wide association analysis was conducted using EMMAX software.The results indicated that the distribution of oleic acid content in the associated populations presented a continuous bimodal pattern and was controlled by two major gene loci.GW AS analysis identified a dominant QTL locus regulating oleic acid content,situated between the 53853975 and 57808820 bases of chromosome C03,with a contribution rate of 62.51％.It was correlated with the SNP marker and peak values of chrC03_53853975(A/G)and chrC03_57808820(C/T),and the SNP molecular marker chrC03_56392730(G/T)was closely linked.The corresponding base mutation resulted in polymorphism.When the peak SNP allele was TT,the phenotypic effect value of this trait was 57.93％.This study disclosed for the first time the dominant QTL locus of the C03 chromosome and its SNP molecular marker for the oleic acid content trait of glycole-type rapeseed,with a high contribution rate,provi-ding a crucial theoretical basis for the efficient improvement of this trait and marker-assisted selection.
关键词
甘蓝型油菜/油酸含量/QTL/SNP分子标记Key words
Brassica napus/oleic acid content/QTL/SNP molecular marker引用本文复制引用
基金项目
贵州省科技支撑计划农业领域重点项目(黔科合支撑[2022]重点031)
贵州喀斯特山区重要作物生物育种平台建设(黔科合中引地[2023]033)
贵州省育种科研基础平台创新能力建设(黔科合服企[2022]014)
黔农科院青年科技基金[2021]07号()
&&(黔农科种质资源[2023]15号)
出版年
2024
国家科技期刊平台
NSTL
万方数据