白魔芋组培快繁体系建立
Establishment of Tissue Culture and Rapid Propagation System of Amorphophallus albus
陈绪言 1陆毅 1郝国歉1
作者信息
- 1. 宜宾学院农林与食品工程学部,四川宜宾 644000
- 折叠
摘要
为了建立四川屏山县白魔芋(Amorphophallus albus)的组培快繁体系,通过正交实验探究白魔芋不同部位外植体、不同浓度植物生长调节剂对快繁体系的影响.结果表明,以经过控制选择得到的刚开始伸出鳞片叶片作外植体,用75％乙醇消毒30 s,再在0.1％HgCl2溶液中泡8 min,最后用无菌水将试剂洗脱,外植体有86.4％存活;最佳愈伤组织诱导及继代增殖培养基为MS+0.1 mg/L NAA+2.0 mg/L BA+25.0 g/L蔗糖+6.0 g/L琼脂,诱导率为87.5％;最佳芽分化及增殖培养基为MS+0.3 mg/L NAA+2.0 mg/L 6-BA+25.0 g/L蔗糖+6.0 g/L琼脂,运用这一培养基不定芽有61.6％成功被诱导,单位接种愈伤组织有3枚以上成功分化出不定芽;最佳诱导生根培养基为1/2 MS+0.1 mg/L NAA+0.6 mg/L BA+25.0 g/L蔗糖+6.0 g/L琼脂,生根率为83.4％,平均根数2.70.最佳外植体为刚开始伸出鳞片3～10 d的幼嫩芽.
Abstract
In order to establish tissue culture and rapid propagation system of Amorphophallus albus in Pingshan County,Sichuan Province.The effects of different parts of Amorphophallus albus explants and different concentrations of plant growth regulators on the rapid propagation system were investi-gated by orthogonal experiment.The results showed that 86.4％of the explants survived after being disinfected with 75％alcohol for 30 seconds,then soaked in 0.1％HgCl2 solution for 8 minutes,and eluted with sterile water.The best culture medium for callus induction and subproliferation was MS+0.1 mg/L NAA+2.0 mg/L BA+25.0 g/L sucrose+6.0 g/L AGAR,the induction rate was 87.5％.The optimal medium for bud differentiation and proliferation was MS+0.3 mg/L NAA+2.0 mg/L 6-BA+25.0 g/L sucrose+6.0 g/L AGAR,which was used for bud adventification and 61.6％of the callus were successfully induced,and more than 3 of the callus were successfully differentiated.The optimal rooting medium was 1/2 MS+0.1 mg/L NAA+0.6 mg/L BA+25.0 g/L sucrose+6.0 g/L AGAR.The rooting rate was 83.4％and the average number of roots was 2.70.The best explants were the young buds that began to extend their scales for 3 to 10 days.
关键词
白魔芋/组织培养/快速繁殖体系Key words
Amorphophallus albus/tissue culture/rapid propagation system引用本文复制引用
基金项目
国家自然科学基金(31700164)
中央引导地方科技发展专项资金项目(2022ZYD02)
国家级大学生创新创业训练计划项目(202410641048)
四川省大学生创新创业训练计划项目(S202310641176X)
出版年
2024
国家科技期刊平台
NSTL
万方数据