Establishment of Tissue Culture and Rapid Propagation System of Amorphophallus albus
In order to establish tissue culture and rapid propagation system of Amorphophallus albus in Pingshan County,Sichuan Province.The effects of different parts of Amorphophallus albus explants and different concentrations of plant growth regulators on the rapid propagation system were investi-gated by orthogonal experiment.The results showed that 86.4％of the explants survived after being disinfected with 75％alcohol for 30 seconds,then soaked in 0.1％HgCl2 solution for 8 minutes,and eluted with sterile water.The best culture medium for callus induction and subproliferation was MS+0.1 mg/L NAA+2.0 mg/L BA+25.0 g/L sucrose+6.0 g/L AGAR,the induction rate was 87.5％.The optimal medium for bud differentiation and proliferation was MS+0.3 mg/L NAA+2.0 mg/L 6-BA+25.0 g/L sucrose+6.0 g/L AGAR,which was used for bud adventification and 61.6％of the callus were successfully induced,and more than 3 of the callus were successfully differentiated.The optimal rooting medium was 1/2 MS+0.1 mg/L NAA+0.6 mg/L BA+25.0 g/L sucrose+6.0 g/L AGAR.The rooting rate was 83.4％and the average number of roots was 2.70.The best explants were the young buds that began to extend their scales for 3 to 10 days.
Amorphophallus albustissue culturerapid propagation system
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