Study on in Vitro Flowering Technology of Cymbidium Plant Cymbidium nanulum
In order to protect,develop and utilize the new Cymbidium plant Cymbidium nanulum and accelerate the breeding process,this study induced in vitro flowering by direct rhizomes and indirect regenerated plants.The results showed that the highest number of normal flowers were obtained by direct induction on the adjusted MS medium containing 1 mg/L 6-BA,30 g/L sucrose and 2 g/L pep-tone(165 mg/L NH4NO3,380 mg/L KNO3 and 850 mg/L KH2PO4),with an average of 2.6.MS or 1/2 MS medium containing 3 mg/L 6-BA,1 mg/L NAA and 100 mL/L coconut water was the most suitable medium for regeneration of Cymbidium nanulum rhizomes.In MS medium containing 3 mg/L 6-BA and 20 g/L sucrose,the number of flowers obtained by indirect induction was the highest,with an average of 0.8.
NETL
NSTL
万方数据
