Establishment of a Culture System for Mature Embryos of Prunus triloba Lindl.
Using mature embryos of Prunus triloba Lindl.as explants,a stable and efficient embryo culture seedling system was established.The germination rate,average plant height,rooting rate,average root length,callus rate and leaf morphology were determined,and MS,1/2 MS and WPM were set.The optimum medium for the seedling cultured from Prunus triloba Lindl.embryo was selected.The germination and rooting tests of embryos were carried out using plant growth regulators 6-BA,NAA,IBA and activated carbon.IBA and NAA levels were 0.05,0.10,0.20 mg/L and 6-BA setting 1 and 2 mg/L 2 levels,the germination and rooting of embryos were counted,and the best medium type of the seedling system was screened.On the basis of the most suitable growth medium for Prunus triloba Lindl.embrasure culture,callus induction and differentiation experiments were conducted at different low temperature treatment times(0,20,40,60,80 days),and callus rate and leaf morphology were collected.Finally,the effects of different degrees of cotyledon retention(com-plete cotyledon,1/2 cotyledon and 1/4 cotyledon)on germination and rooting of embryo culture were analyzed.The results showed that MS medium was the most suitable medium for the germination and epicotyl growth of Prunus triloba Lindl.embryo.The germination rate and rooting rate were the highest on MS+6-BA 1.0 mg/L+IBA 0.10 mg/L+NAA 0.10 mg/L+activated carbon 500 mg/L,which were 83.33％and 86.67％,respectively.The most suitable time for Prunus triloba Lindl.embryo development and normal growth was refrigerated at 4 ℃ from 60 to 80 days,and when the refrigeration time was 80 days,the germination rate reached 100％,the rooting rate was 93.33％,and the leaves were green and flat.Keeping intact cotyledon was more conducive to promoting the elonga-tion of epicotyl,and keeping 1/4 cotyledon was more conducive to promoting the elongation of hypo-cotyl.In this study,the most suitable seedling medium was MS+6-BA 1.0 mg/L+IBA 0.10 mg/L+NAA 0.10 mg/L+activated carbon 500 mg/L.
Prunus triloba Lindl.embryo culturetype of culture mediumplant growth regulatorslow temperature treatmentdegree of cotyledon retention
万方数据
维普
