种子2024,Vol.43Issue(8) :45-51,59.DOI:10.16590/j.cnki.1001-4705.2024.08.045

倒刺狗尾草叶绿体基因组特征及系统发育分析

Analysis on the Characteristics and Phylogeny of Chloroplast Genome of Setaria verticillata

刁玉霖 李竑阳 张海金 张文飞 王凯玺 朱康宁 张蒙 孙鑫 李前 陈国秋
种子2024,Vol.43Issue(8) :45-51,59.DOI:10.16590/j.cnki.1001-4705.2024.08.045

倒刺狗尾草叶绿体基因组特征及系统发育分析

Analysis on the Characteristics and Phylogeny of Chloroplast Genome of Setaria verticillata

刁玉霖 1李竑阳 2张海金 1张文飞 1王凯玺 1朱康宁 1张蒙 1孙鑫 1李前 1陈国秋1
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作者信息

  • 1. 辽宁省旱地农林研究所,辽宁朝阳 122000
  • 2. 云南省玉溪市农业技术推广站,云南玉溪 653100
  • 折叠

摘要

为了对倒刺狗尾草叶绿体基因组的基本特征和系统发育进行分析,本研究对倒刺狗尾草的叶绿体基因组进行了测序,并对测序结果进行组装、注释和特征分析,并以拟南芥为外类群,利用15种已知叶绿体基因组的禾本科植物构建系统发育树.结果表明,倒刺狗尾草叶绿体基因组总长度为138 946 bp,总GC含量为38.5%,共注释得到131个基因,包括83个CDS基因、40个tRNA基因和8个rRNA基因.密码子偏向使用A/T结尾的密码子.倒刺狗尾草检测到60个简单重复序列(SSR),A-T重复较多.通过系统发育分析得知,倒刺狗尾草与蒺藜草亲缘关系最为密切.

Abstract

In order to analyze the basic characteristics and phylogeny of the chloroplast genome of Setaria verticillata,in this study the chloroplast genome of Setaria verticillata was sequenced,with the sequencing results assembled,annotated and characteristics analysed.In addition,15 species of grasses with known chloroplast genomes were used to construct phylogenetic trees with Arabidopsis thaliana as exotaxa.The results showed that the total length of chloroplast genome was 138 946 bp,the total GC content was 38.5%,and a total of 131 genes were annotated,including 83 CDS genes,40 tRNA genes and 8 rRNA genes.Codons tend to use codons ending in A/T.60 simple repeat sequences(SSRS)were detected in Setaria verticillata,and A-T repeats were more frequent.Phylogenetic analysis showed that Setaria verticillata was most closely related to Cenchrus echinatus.

关键词

倒刺狗尾草/叶绿体基因组/系统发育

Key words

Setaria verticillata/chloroplast genome/phylogeny

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基金项目

国家现代农业产业技术体系(CARS-06-14.5-B15)

辽宁省民生计划项目(2021JH2/10200029)

辽宁省建平县杂粮产业乡村振兴科技特派团(2023020322-JH5/104)

谷子产业提升技术示范推广(2024-XCZX-A2-72)

辽宁省农业科学院院长基金项目(2024QN1813)

出版年

2024
种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子:0.502
ISSN:1001-4705
参考文献量28
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