Transcriptome Dynamic Analysis on Young Panicle of Rice at Booting Stage Under Cold Stress
In order to further understand the mechanism of low temperature tolerance in booting stage of rice(Oryza sativa)in cold regions and clarify the molecular mechanism of low temperature response,the transcriptome dynamics of young ears of Longjing25,a rice variety with strong cold tolerance at booting stage,was analyzed.A total of 515.26 Mb of Clean Reads were obtained by sequencing,and 6 927 differentially expressed genes(DEGs)were screened.Among them,6 784 DEGs were obtained after 2 days low temperature treatment.After four days of low temperature treatment,DEGs decreased significantly and only 1 149 were obtained.The GO enrichment analysis showed that the up-regulated and down-regulated genes in each part of the Venn diagram were significantly enriched into 33 biological process GO categories,among which the category containing the largest number of genes was the single biological metabolic process,and the low-temperature response and high-temperature response were the most enriched in the Venn diagram of LG25.KEGG enrichment analysis showed that DEGs were enriched to 32 signal pathways in all parts of the Venn Diagram,among which plant hormone signal transduction pathways were the most significantly enriched in all parts of the Venn diagram,including 83 DEGs was distributed in 8 hormone pathways including abscisic acid,auxin,brassinolide,cytokinin,gibberellin,ethylene,jasmonic acid and salicylic acid.Auxin,abscisic acid and salicylic acid contained more DEGs(26,17 and 15,respectively).
rice in cold regionsreproductive stagecold temperturetranscriptomeplant hormones