In this study,using Ricinus communis L.as research object,the Ricinus communis L.Ole-osin gene family was comprehensively identified and analyzed by bioinformatics methods.Five mem-bers of Oleosin gene family with sequence length ranging from 138 to 232 aa were obtained.The mo-lecular weight ranged from 14.484 93 to 25.424 61 kDa.The isoelectric point was between 9.07 and 10.96;They were hydrophobic proteins with highly conserved proline junctions.α-helix and random curl-ing were the main components of Ricinus communis L.Oleosin.Motif1 to Motif4 were found in all Ricinus communis L.Oleosin proteins by motif analysis.The five Oleosin genes encoding these pro-teins were distributed on four chromosomes and were divided into three subfamilies,U-Oleosin,SL-Oleosin and SH-Oleosin.Gene structure analysis showed that all RcOleosin genes had no intron except RcOleosin2.There were multiple cis-acting elements related to stress response in all five gene promot-er regions.Collinear analysis showed that genome polyploidy was not the main reason for the expan-sion of Ricinus communis L.Oleosin gene family.The transcriptome data of Ricinus communis L.seeds under salt-alkali stress showed that all five Oleosin genes had certain regulatory functions during salt-alkali stress.
关键词
蓖麻/油体蛋白/基因家族/生物信息学
Key words
Ricinus communis L./Oleosin/gene family/bioinformatics
维普
万方数据