首页|蓖麻油体蛋白基因家族鉴定和盐碱胁迫响应分析

蓖麻油体蛋白基因家族鉴定和盐碱胁迫响应分析

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本研究以蓖麻为对象,利用生物信息学手段对蓖麻Oleosin基因家族进行全面鉴定分析,得到5个Oleosin基因家族成员,序列长度在138~232 aa之间;分子量在14.484 93~25.42461 kDa之间;等电点介于9.07~10.96之间;均为疏水性蛋白且具有高度保守的"脯氨酸结";α-螺旋和无规则卷曲是蓖麻Oleosin蛋白的主要组成结构;基序分析显示,Motif1~Motif4存在所有蓖麻Oleosin蛋白中.编码这些蛋白的5个Oleosin基因分布于4条染色体上,在进化关系上被划分到U-Oleosin、SL-Oleosin和SH-Oleosin三个亚族;基因结构分析显示,除RcOleosin2有一个内含子外,其余RcOleosin基因均无内含子;5个基因启动子区域均存在多种与逆境响应相关的顺式作用元件;共线性分析显示,基因组多倍化不是蓖麻Oleosin基因家族扩张的主要原因;盐碱胁迫下的蓖麻种子转录组数据表明,5个Oleosin基因均在盐碱胁迫过程中有一定的调节功能.
Identification for Oleosin Gene Family and Analysis on Its Saline and Alkaline Stress Response in Ricinus communis L.
In this study,using Ricinus communis L.as research object,the Ricinus communis L.Ole-osin gene family was comprehensively identified and analyzed by bioinformatics methods.Five mem-bers of Oleosin gene family with sequence length ranging from 138 to 232 aa were obtained.The mo-lecular weight ranged from 14.484 93 to 25.424 61 kDa.The isoelectric point was between 9.07 and 10.96;They were hydrophobic proteins with highly conserved proline junctions.α-helix and random curl-ing were the main components of Ricinus communis L.Oleosin.Motif1 to Motif4 were found in all Ricinus communis L.Oleosin proteins by motif analysis.The five Oleosin genes encoding these pro-teins were distributed on four chromosomes and were divided into three subfamilies,U-Oleosin,SL-Oleosin and SH-Oleosin.Gene structure analysis showed that all RcOleosin genes had no intron except RcOleosin2.There were multiple cis-acting elements related to stress response in all five gene promot-er regions.Collinear analysis showed that genome polyploidy was not the main reason for the expan-sion of Ricinus communis L.Oleosin gene family.The transcriptome data of Ricinus communis L.seeds under salt-alkali stress showed that all five Oleosin genes had certain regulatory functions during salt-alkali stress.

Ricinus communis L.Oleosingene familybioinformatics

何汉琼、姜洪进、张文婧、马钰雯、孟子轩、李月明、邓晓霞、蔺吉祥

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山西农业大学资源环境学院,太原 030031

东北林业大学园林学院,哈尔滨 150040

蓖麻 油体蛋白 基因家族 生物信息学

山西农业大学校科技创新提升工程项目

CXGC2023027

2024

种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子:0.502
ISSN:1001-4705
年,卷(期):2024.43(8)