Electronic Cloning and Bioinformatics Analysis of ThPLA Gene in Thinopyrum elongatum
In this study,the phospholipase gene(ThPLA)of Thinopyrum elongatum was obtained by electronic cloning method based on high-throughput sequencing information.Combined with bioinfor-matics analysis methods,the amino acid physicochemical properties,conserved domain,advanced structure,hydrophilic/hydrophobic,transmembrane domain,homology analysis and functional ex-pression of the gene gDNA and coding protein were predicted and analyzed.It was found that the total length of ThPLA gene was 1 620 bp,encoding 539 amino acids,of which the PLA protein was most closely related to Aegilops tauschii Coss.,Triticum turgidum var.dicoccoides,Oryza sativa L.and Zea mays L..Subcellular localization and GO term predicted that ThPLA protein was located in chlo-roplasts.Combining the results of gene expression prediction and transcriptome data,the gene could respond to salt stress in plant roots.
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