首页|新疆小麦地方品种重要功能基因的KASP标记检测

新疆小麦地方品种重要功能基因的KASP标记检测

扫码查看
原文链接
  • 万方数据
  • 维普
本研究利用10种与表型性状有关的44个高通量KASP(Kompetitive allele specific PCR)分型标记技术,检测了117份新疆小麦地方品种的表型、品质、面粉色泽、籽粒蛋白质含量、粒重、抗逆、抗病等相关基因.结果表明,Ppd-D1光周期位点检测到非敏感材料116份.春化、矮杆基因位点检测中,冬性材料比例97％以上,矮化基因比例96％以上.Glu-A3和Glu-B3的标记检测中,高筋材料的比例仅占1％.有利等位基因Psy-B1中被检测出低黄色素材料,占95.7％;Pds-B1和Psy-A1基因中,以高黄色素材料为主.NAM-A1检测出品质相关A1b、A1d类型材料116份.GCP_DUP标记鉴定携带低分子量谷蛋白亚基的材料占97％以上.抗病相关基因中,以抗叶锈病的材料为主.113份材料检测为抗穗发芽类型.
Detection of Important Functional Genes in Xinjiang Local Wheat Cultivars by KASP Markers
This study using 10 kinds of phenotypic traits about 44 high-throughput KASP(kompetitive allele specific PCR)type markers,the phenotype,quality,flour colour and lustre,grain protein con-tent,grain weight,stress resistance,disease resistance genes of 117 Xinjiang local wheat(Triticum aestivum L.)cultivars were tested.The results showed that 116 non-sensitive materials were detected at the photoperiod site of Ppd-D1.In the detection of vernalization and dwarf gene loci,the proportion of winter materials was more than 97％,and the proportion of dwarfing genes was more than 96％.In the detection of Glu-A3 and Glu-B3 labeling,the proportion of high reinforcement materials accounted for only 1％.Low yellow pigment material was detected in the favorable allele Psy-B1,accounting for 95.7％;Pds-B1 and Psy-A1 genes were mainly high yellow pigment materials.NAM-A1 detected 116 quality related A1b and A1d materials.More than 97％of materials carrying low molecular weight glutenin subunits were identified by GCP_DUP labeling.Among the genes related to disease resist-ance,the materials resistant to leaf rust were the main ones.113 materials were found to be anti-sprouting types.

local wheat cultivarsfunctional geneKASP marker

哈力旦·依克热木、刘娜、刘联正、周安定、曹俊梅、张新忠

展开 >

新疆农业科学院粮食作物研究所,乌鲁木齐 830091

农业农村部西北地区小麦抗旱耐盐生物学科学观测实验站,乌鲁木齐 830091

新疆小麦产业技术体系乌鲁木齐综合试验站,乌鲁木齐 830091

小麦地方品种 功能基因 KASP标记

新疆维吾尔自治区重大科技专项项目新疆维吾尔自治区重大科技专项项目新疆维吾尔自治区重大科技专项项目新疆维吾尔自治区自然科学基金面上项目科技创新2030—重大项目子课题农业农村部盐碱土改良与利用(干旱半干旱区盐碱地)重点实验室基金课题农业科技创新稳定支持项目自治区公益性科研院所基本科研业务经费资助项目新疆农业科学院青年科技骨干创新能力培养项目

2022A02003-12022A03004-32021A02001-12023D01A942023ZD0402602YJDKFJJ202304xjnkywdzc-2023001-24KY2023029xjnkq-2023001

2024

10.16590/j.cnki.1001-4705.2024.08.126

种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子：0.502
ISSN：1001-4705
年,卷(期)：2024.43(8)