Different Pollination Methods on Pollen Germination and Pollen Tube Growth in Musa nana and Fluorescence Microscope
The flower,ovary and ovule structure of banana were observed with wild Musa nana germplasm resource Musa acuminata Colla of fertile and high-seed setting(AA)and Musa × paradisiaca L.cultivar'Musa Pisang Awak subgroup'(ABB),and the effects of different pollination methods on pollen germination and pollen tube growth were studied.The results showed that the pollen germination rate of Musa acuminata Colla was high after self-pollination,and the germination quantity increased with pollination time,reached the peak at 3 days after pollination,and did not change after pollination.The pollen tube grew rapidly,reaching the middle of the style 8 hours after pollination,reaching the ovary 24 hours after pollination,and the fertili-zation process lasted from 1 to 3 days.Under different pollination methods,the germination quantity of self-pollinated pollen was significantly higher than that of cross-pollination,and the germination peak was 2 days earlier than that of cross-pollination.Moreover,cross-pollinated pollen tubes could produce deformities,such as short and thick pollen tubes,thick and swollen ends,and distorted shapes.In summary,wild Musa nana germplasm resources were self-compatible,pollen germination,pollen tube elongation and fertilization process were well developed,which was an ideal hybrid material.The efficiency of cross breeding was low due to the decreased compatibility between pollen and stigma,abnormal pollen tube germination and incomplete ovule development of cultivated species.
万方数据
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