首页|地盘松转录组密码子偏好性分析和直系同源基因鉴定

地盘松转录组密码子偏好性分析和直系同源基因鉴定

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地盘松是云南松的一个灌木状变种,其生长习性与常见的松属植物有着显著的区别.本研究旨在探究地盘松的遗传联系和遗传规律,通过对地盘松转录组进行密码子偏好性分析、直系同源基因鉴定和选择压力分析,深入了解地盘松的演化和适应策略.密码子偏好性分析揭示了地盘松基因信息翻译过程中的重要规律,而直系同源基因鉴定为研究地盘松的遗传特征和进化历程提供了重要信息.结果表明,地盘松、马尾松和油松在CDS密码子第二位和第三位偏好A/T碱基,且在第三位碱基使用上具有偏向性,其中T和G的使用频率较高.不同松树种类在密码子的使用上存在一定差异,但整体展现出类似的趋势.通过直系同源基因鉴定发现,地盘松蛋白质组中存在多拷贝基因,并且部分基因可能受到正选择压力的影响,如生长素合成、响应和转运等基因.
Analysis on Transcriptome Codon Preference and Identification of Immediate Homologous Genes in Pinus yunnanensis var.pygmaea
Pinus yunnanensis var.pygmaea is a shrubby variety of Pinus yunnanensis,and its growth habits are significantly different from those of common Pinus plants.The aim of this study was to explore the genetic links and rules of Pinus yunnanensis var.pygmaea,and to understand the evolu-tion and adaptation strategies of Pinus yunnanensis var.pygmaea through codon preference analysis,direct homologous gene identification and selection pressure analysis of the transcriptome of Pinus yunnanensis var.pygmaea.Codon preference analysis revealed important rules in the process of genetic information translation,and the identification of orthologous genes provided important infor-mation for the study of genetic characteristics and evolutionary history of Pinus yunnanensis var.pygmaea.The results showed that the second and third positions of the CDS codon of Pinus yun-nanensis var.pygmaea,Pinus massoniana Lamb.and Pinus tabuliformis Carrière preferred A/T base,and there was a bias in the use of the third base,in which T and G were used more frequently.The codon usage of different pine species was different to some extent,but the general trend was sim-ilar.Direct homologous gene identification showed that there were multiple copies of the proteome,and some of the genes could be affected by positive selection pressure,such as auxin synthesis,response and transport genes.

Pinus yunnanensis var.pygmaeacodon preferenceimmediate homologous genesselection pressure analysis

杨耀、赵杨、王秀荣、肖枫、刘富华

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贵州大学林学院/贵州省森林资源与环境研究中心,贵阳 550025

地盘松 密码子偏好性 直系同源基因 选择压力分析

国家自然科学地区科学基金项目

32060353

2024

种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子:0.502
ISSN:1001-4705
年,卷(期):2024.43(9)
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