In order to improve the TMV resistance of flue-cured tobacco(Nicotiana tabacum L.)varie-ty Yunongxiang201,using Yunongxiang201 as modified materials,a TMV immune homozygous strain YT01 was obtained by hybridization,backcross and phenotypic screening with molecular mark-er and TMV immune variety Va770 as the parent.The agronomic characters and field growth period of YT01 were basically consistent with those of Yunongxiang201.The results showed that the resist-ance of YT01 to TMV was immunity,the accumulation of reactive oxygen species after TMV treat-ment was lower than that of Yunongxiang201,and the positive TMV regulatory genes NtRAR1,NtNTF4,NtPR1a,NtHAK1,NtSGT1 in leaves,of which the relative expression of TMV CP was significantly higher than that of Yunongxiang201,and the relative expression of TMV CP was signifi-cantly lower than that of Yunongxiang201.The main chemical composition was basically consistent with that of Yunongxiang201.The appearance quality and sensory quality were slightly better than Yunongxiang201.The results showed that the modified strain YT01 was immune to TMV and other traits was basically consistent,and its quality was improved,which provided the material basis for the resistance of Yunongxiang201 to virus disease.
维普
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