Establishment of Tissue Culture and Regeneration System for F1 Hybrid of Agropyron mongolicum Keng×Agropyron cristatum cv.Fariway
The F1 young panicle of the interspecific hybrid of Agropyron mongolicum Keng × Agropy-ron cristatum cv.Fariway was selected as explants to screen the hormone concentration affecting cal-lus induction,differentiation and rooting,and a complete plant regeneration system was established.The results showed that the optimal callus induction medium of hybrid F1 generation was MS+2,4-dichlorophenoxyacetic acid(2,4-D)1.5 mg/L+6-benzylaminopurine(6-BA)0.3 mg/L,and the heal-ing rate was 81.7%.The medium for callus differentiation was 6-BA 2.5 mg/L+naphthalene acetic acid(NAA)0.4 mg/L,and the differentiation rate was 71.7%.The rooting medium was 1/2 MS+NAA 0.2 mg/L,and the rooting rate was 88.3%.After refining the seedlings,the survival rate of each substrate transplanting was more than 90%.In this study,the F1 generation tissue culture regeneration system of Agropyron mongolicum Keng × Agropyron cristatum cv.Fariway hybrid was established to effectively preserve the F1 generation and laid a foundation for the recovery of chro-mosome doubling induced by colchicine in the later stage.
Agropyron mongolicum KengAgropyron cristatum cv.Fariwayhybrid F1young inflorescenceregeneration system