Establishment of Tissue Culture and Rapid Propagation System of Rubus corchorifolius
With red Rubus corchorifolius,black Rubus corchorifolius and Rubus chingii Hu as experi-mental materials,stem segments,stem tips and leaves were selected as explants to establish the rapid propagation system of tissue culture of Rubus corchorifolius.The results showed that the best explants for obtaining the inoculum were stem segment and stem tip,and the best medium for indu-cing Rubus corchorifolius cluster buds was MS+6-BA(1.0-2.0)mg/L+NAA(0.1-0.2)mg/L.The best callus budding medium was MS+6-BA(5.0 mg/L)+NAA(0.2 mg/L);The medium of red Rubus corchorifolius proliferation culture:MS+6-BA(0.5-1.0)mg/L+NAA(0.2 mg/L);The growth medium of black Rubus corchorifolius was MS+6-BA(1.0 mg/L)+NAA(0.05-0.1)mg/L;The medium for the proliferation of Rubus chingii Hu was MS+6-BA(1.0 mg/L)+NAA(0.2 mg/L).Rooting was induced by 1/2 MS+IBA(1.0 mg/L)medium.The cultivation medium was carried out in Vclay to Vpeat soil=1∶1 under 0.05％KMnO4 sterilization condition.
万方数据
维普
