Identification of Key Modules and Candidate Genes for Seed Aging Resistance of Oryza sativa subsp.indica Kato.
Seeds have to go through the aging process since they are mature,which leads to the irre-versible decline or even the complete loss of their vitality,causing great losses to agricultural produc-tion.The Oryza sativa subsp.indica Kato.had stronger storage resistance than Oryza sativa subsp.japonica Kato..Although some anti-aging genes have been cloned,the key genes involved in the anti-aging process of indica rice seeds and their regulatory mechanisms remained unclear.In this study,transcriptomic analysis was performed on kasalath genotype rice seeds(Oryza sativa subsp.indica Kato.)at 8 aging stages(0,4,8,10,12,15,22,28 days).The results showed that the gene expres-sion level decreased gradually with the aging time,and the overall FPKM difference was not signifi-cant.A total of 6 517 differentially expressed genes were identified,and KEGG enrichment analysis showed that differentially expressed genes involved in metabolic pathways such as spliceosome,DNA replication,base excision repair,ribosome,endoplasmic reticulum protein processing,and ubiquitin-mediated protein hydrolysis were significantly enriched.Weighted gene co-expression network analy-sis,WGCNA gene were generated a total of 24 modules,of which turquoise,blue,gray and brown modules significantly correlated with seed aging stage.These modules were involved in the regulation of nucleic acid,protein damage repair systems and soluble sugars.In addition,75 central genes were identified,including OsLTPL67,OsERF-1,OsLEA4,OsSMP,OsGRX2,OsDOG1L-3 and OsGRX14,which played important roles in the aging network of rice seeds.These findings provided a broader perspective for studying the regulatory mechanisms of seed aging and provided a wealth of potential target genes for rice breeding.
Oryza sativa subsp.indica Kato.spliceosomeembryonic developmentribosomeseed longevityreactive oxygen species
万方数据
维普
