Study on Genetic Diversity of Miao Medicine Ardisia crenata Root Based on ISSR Molecular Markers
In order to explore the genetic diversity level and relationship of different populations of Ardisia crenata,24 different populations of Ardisia crenata were selected and analyzed by ISSR molecular marker technology.The results showed that 14 primers with good amplification effect and high polymorphism were selected from 100 primers,and genetic diversity was detected in 24 popula-tions of Ardisia crenata.There were 75 amplification sites detected,with an average of 5.4 amplifica-tion sites per primer,74 polymorphic sites,and polymorphism ratio(PPB)was 98.67％.UBC841 had the most amplification sites(8),and UBC820,UBC821,UBC844 and UBC887 had the least amplifi-cation sites(4).The observed number of alleles was 2,the effective number of alleles was 1.086 8-2.000 0,the gene diversity of Nei's was 0.079 9-0.500 0,and the Shannon's index was the largest(0.693 1).The correlation analysis between genetic distance and geographical distance were found no significant correlation.Study showed that the genetic diversity of Ardisia crenata germplasm resources was high,and ISSR molecular markers could be used to identify the relatives of Ardisia crenata.
万方数据
维普
