Development of KASP Markers for Sorghum Mitochondrial Male Sterility Genes
In order to shorten breeding life,improve breeding efficiency and create new germplasm,96 male sterile sorghum Sorghum bicolor(L.)Moench lines,maintainers and restorer lines were used as materials in this study.Through mitochondrial resequencing technology and Chi-square test of pheno-typic data and SNP sites,SNP sites related to fertility were discovered,and KASP molecular marker sites were developed.The results showed that 434 SNP sites related to fertility were identified,and 101 SNP sites were selected for Chi-square test.SNPS x2 was 9.574,p values was 0.002,and the mutation sites were mainly transposition,and the overall Tv/Ts was 1.55.49 materials containing sterile genes and 47 fertile genotypes were identified.The selected SNP sites openly emitted 19 pairs of high-quality KASP primers,and these markers could be used to accurately distinguish the sorghum sterile line from the maintainer line,and identify whether the restorer line contained plasmid male sterility genes.Developing these SNPS into molecular markers would result in significant labor and time savings and improved accuracy during breeding.
sorghummitochondriacytoplasmic-nuclear male sterilitycompetitive allele
万方数据
维普
