In order to reveal the quantitative relationship between seed vitality and TTC staining,this study took rice(Oryza sativa L.)seed CY-150 as the experimental material.After artificial acceler-ated aging treatment and TTC staining,stereoscopic RGB images of the longitudinal section of seed embryo were obtained,and the CIELab values of the images were analyzed by the image analysis soft-ware established by the Plant New Variety Testing(Kunming)Sub-center of the Ministry of Agricul-ture and Rural Affairs,and calculating the staining intensity per unit.The results showed that there was a significant positive correlation between germination rate,TTC staining rate and vigor index.Among them,the correlations between germination rate and vitality index,germination rate and stai-ning rate,staining rate and vitality index were 0.997 6,0.971 8 and 0.963 1.In addition,CIELab value was significantly correlated with germination rate,staining rate and vitality index,in which L*and a*were negatively correlated and positively correlated,and Pearson correlation coefficients were-0.974 9,-0.976 1,-0.972 1,0.973 7,0.991 5 and 0.967 8,respectively.At the same time,the seed vigor equation was established using seed vigor index and CIELab value.Four rice varieties were randomly selected to verify the equation,and the results showed that the real values of the vigour index of the four varieties were slightly higher than the predicted values.The results showed that TTC staining could not only be used for rapid qualitative analysis of rice seed viability,but also could be used for quantitative analysis of seed viability through seed embryo RGB images,which provided a reference for further popularization of rapid detection of rice seed viability.
维普
万方数据
