伏牛山野生黄精组培快繁体系建立及组培苗质量评估

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中文摘要：以伏牛山野生黄精的种子为试验材料,建立其组培快繁体系并对组培苗质量进行评估.结果表明,伏牛山野生黄精种子萌发最佳培养基为1/2 MS+0.5 mg/L ABA,丛生芽诱导最佳培养基为MS+4.0 mg/L 6-BA+0.2 mg/L NAA+0.5 mg/L GA3,丛生芽增殖最佳培养基为 MS+3 mg/L 6-BA+0.1 mg/L TDZ+0.5 mg/L 2,4-D+0.4 mg/L NAA,生根最佳培养基为1/2 MS+0.5 mg/L NAA+0.2 mg/L IBA;组培苗分级标准为Ⅰ级苗:株高≥4.2 cm、叶片≥5个,Ⅱ级苗:3 cm＜株高＜4.2 cm、2＜叶片数＜5,Ⅲ级苗株高≤3 cm、叶片数≤2个,移栽后成活率为88％.

外文标题：Establishment of a Tissue Culture System with Quality Assessment for Wild Polygonatum sibiricum in Funiu Mountain

外文摘要：The tissue culture rapid propagation system was established and the quality of the tissue culture seedlings was evaluated with the wild Polygonatum sibiricum seeds from Funiu Mountain as the research material.The results showed that the best medium for seed germination of Polygonatum sibiricum from Funiu Mountain was 1/2 MS+0.5 mg/L ABA,the best medium for cluster bud induc-tion was MS+4.0 mg/L 6-BA+0.2 mg/L NAA+0.5 mg/L GA3,and the best medium for cluster bud proliferation was MS+3 mg/L 6-BA+0.1 mg/L TDZ+0.5 mg/L 2,4-D+0.4 mg/L NAA,the best rooting medium was 1/2 MS+0.5 mg/L NAA+0.2 mg/L IBA.The classification standard of tissue culture seedlings was Grade Ⅰ:plant height ≥4.2 cm,leaves ≥5,Grade 11:3 cm＜plant height＜4.2 cm,2＜number of leaves＜5,Grade m:plant height ≤3 cm,number of leaves ≤2,the survival rate after transplantation was 88％.

外文关键词：

Polygonatum cyrtonemaseedtissue culturequality of tissue culture seedlings

作者：

董薇、余永亮、许兰杰、杨红旗、安素妨、杨青、苏小雨、梁慧珍

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作者单位：

河南省农业科学院中药材研究所,郑州 450002

关键词：

黄精种子组培组培苗质量

基金：

财政部和农业农村部:国家现代农业产业技术体系河南省重大科技专项河南省重点研发专项中央本级重大增减支项目河南省农科院自主创新专项基金河南省科技攻关项目河南省科技攻关项目河南省科技攻关项目河南省农业科学院优秀青年科技基金

项目编号：

CARS-2122110031040023111111080020603022023ZC0832321021102622421023105312421021102482024YQ16

出版年：

2024

DOI：

10.16590/j.cnki.1001-4705.2024.10.142

种子

贵州省种子管理站贵州省种子学会中国种子协会

种子

CSTPCD北大核心

影响因子：0.502

ISSN：1001-4705

年,卷(期)：2024.43(10)