The tissue culture rapid propagation system was established and the quality of the tissue culture seedlings was evaluated with the wild Polygonatum sibiricum seeds from Funiu Mountain as the research material.The results showed that the best medium for seed germination of Polygonatum sibiricum from Funiu Mountain was 1/2 MS+0.5 mg/L ABA,the best medium for cluster bud induc-tion was MS+4.0 mg/L 6-BA+0.2 mg/L NAA+0.5 mg/L GA3,and the best medium for cluster bud proliferation was MS+3 mg/L 6-BA+0.1 mg/L TDZ+0.5 mg/L 2,4-D+0.4 mg/L NAA,the best rooting medium was 1/2 MS+0.5 mg/L NAA+0.2 mg/L IBA.The classification standard of tissue culture seedlings was Grade Ⅰ:plant height ≥4.2 cm,leaves ≥5,Grade 11:3 cm＜plant height＜4.2 cm,2＜number of leaves＜5,Grade m:plant height ≤3 cm,number of leaves ≤2,the survival rate after transplantation was 88％.
关键词
黄精/种子/组培/组培苗质量
Key words
Polygonatum cyrtonema/seed/tissue culture/quality of tissue culture seedlings
维普
万方数据