In order to study the rapid propagation technology of Dendrobium fanjingshanensis and pro-vide basic data for its conservation,the stem segment and seed of Dendrobium fanjingshanensis were used as explants to study the rapid propagation technology of tissue culture.The results showed that:with stem segment as explants,the best disinfection methods were:75％ethanol disinfection for 30 s,0.1％HgCl2 disinfection for 13 minutes,and the best combination of axillar bud induction was:1/2 MS/B5+0.5 mg/L 6-BA+0.2 mg/L NAA,the optimal combination of adventitive bud induction and proliferation was:1/2 MS+0.5 mg/L NAA+1 mg/L 6-BA,with seeds as explants,the optimal combination of seed germination and procorm induction was:MS+1.0 mg/L NAA+1.0 mg/L 6-BA+1 g/L activated carbon,and the optimal combination of procorm proliferation was:MS+0.5 mg/L NAA+2 mg/L 6-BA,the optimal proliferation of sterile seedlings was MS+1.0 mg/L NAA+1.0/2.0/3.0 mg/L 6-BA.The best combination of rooting and strong seedlings was MS+0.5 mg/L NAA+1/0.1/0.5 mg/L IBA.
维普
万方数据