Full-length Transcriptome Sequencing and Bioinformatics Analysis of Corydalis saxicola Bunting
In order to obtain full-length transcriptome data of Corydalis saxicola Bunting,full-length transcriptome sequencing was performed using PacBio platform and SMRT technology with 6-month-old seedlings as materials in this study.A total of 14 183 high-quality transcripts with no redundancy were obtained,which were analyzed by NT,NR,BLASTX,BLASTP,eggNOG and GO When com-pared with other databases,100％of the transcripts were successfully annotated.NR annotation results showed that the transcripts of Corydalis saxicola Bunting were associated with Macleaya cordata(Willd.)R.Br.,Tetracentron sinense Oliv.,Papaver californicum.The GO,KOG and KEGG annotations found that these transcripts were mainly involved in biological and metabolic processes.The structural analysis of the transcripts showed that 176 of them had variable shear events(AS),and the intron retention type(RI)was the most common(56.82％).A total of 20 208 CDS were obtained,and most of them were less than 1 000 bp(64.1％).A total of 12 081 SSR loci were detected,including 6 types of single base repeats,two base repeats,three base repeats,four base repeats,five base repeats and six base repeats,among which three base repeats accounted for the highest proportion(41.49％).In addition,194 lncRNAs and 5 590 TFs sequences were predicted,and these transcription factors belonged to the bHLH,MYB-related,NAC family.Further analysis showed that there were 30,22,70,6 and 66 genes involved in the biosynthesis of alkaloids,fla-vonoids,steroids,triterpenoids and nucleosides,respectively.The acquisition and analysis of the full-length transcriptome data of Corydalis saxicola Bunting not only enriched the genetic information of this species,but also provided an important theoretical basis for the study of its adaptation to the environment and the synthesis pathway of medicinal components.
万方数据
维普
