Study on the Identification about Ploidy and SSR Molecular Marker of Interspecific Hybrids between Rehmannia glutinosa and Rehmannia chingii
In order to identify the chromosome ploidy and hybrid authenticity of the hybrid offspring of Rehmannia glutinosa and Rehmannia chingii,the chromosome ploidy was studied by flow cytome-try,and the hybrid authenticity was identified by SSR molecular marker.The results showed that Rehmannia glutinosa'Fenhua'was tetraploid,consistent with the maternal Rehmannia glutinosa,and the paternal Rehmannia chingii was diploid.By PCR,11 pairs of SSR primers were selected from 45 pairs of primers for the identification of hybrid authenticity.The total number of different bands was 45,the number of polymorphic bands was 40,and the polymorphism ratio was 88.89％.Primers were removed except CPSSR_225,and it was found from the amplified maps of other primers that the peak value of genes in Rehmannia glutinosa'Fenhua'was the same as the partial peak value of paternal and maternal genes,and there were 4 peaks in one locus,2 of which were consistent with the maternal and paternal genes respectively.The results showed that Rehmannia glutinosa'Fenhua'was the true hybrid of Rehmannia glutinosa and Rehmannia chingii.
万方数据
维普
