珍稀濒危植物焕镛木种子休眠及萌发特性研究
Research on the Dormancy and Germination Characteristics of the Rare and Endangered Plant Wooyoungia septentrionalis Seeds
靳倩文 1王曾辉 1毛跃雄 2彭洋 2欧静1
作者信息
- 1. 贵州大学林学院,贵阳 550025
- 2. 贵州省国有龙里林场,贵州龙里 551200
- 折叠
摘要
为探究焕镛木种子的休眠原因及萌发特性,寻找解除休眠的方法,以成熟的焕镛木种子为材料,研究种子形态、透水性、内源抑制物、不同处理方式等对焕镛木种子萌发的影响,并测定萌发过程中生理生化指标的变化.结果表明,焕镛木种子种皮不造成透性障碍,不存在物理休眠;假种皮、种皮、胚乳均含有萌发抑制物,且胚极小未完全分化,存在形态生理休眠;250 mg/L的赤霉素(GA3)浸种48 h,焕镛木种子发芽率可达57.58％,25 ℃/15 ℃变温层积90 d是打破焕镛木种子休眠的最佳方法,其发芽率可达60.2％;焕镛木种子萌发过程中可溶性糖含量和可溶性蛋白质含量总体变化显著降低,内源激素赤霉素(GA3)、油菜素甾醇(BR)、玉米素核苷(ZR)、吲哚乙酸(IAA)、茉莉酸(JA)协同促进种子萌发及出苗,脱落酸(ABA)抑制种子萌发.
Abstract
In order to explore the reasons of dormancy and germination characteristics of Wooyoungia septentrionalis seeds and find ways to relieve dormancy,using mature Wooyoungia septentrionalis seeds as materials,the effects of seed morphology,water permeability,endogenous inhibitors and different treatments on germination of Wooyoungia septentrionalis seeds were studied,and the chan-ges of physiological and biochemical indexes during germination were measured.The results showed that the seed coat did not cause permeability barrier and did not have physical dormancy.The arillum,seed coat and endosperm all contained germination inhibitors,and the embryo was very small and not fully differentiated,and there was morphophysiological dormancy.After soaking 250 mg/L of gibberellin(GA3)for 48 hours,the germination rate of Wooyoungia septentrionalis seeds could reach 57.58％.The best method to break the dormancy of Wooyoungia septentrionalis seeds was to layer at 25 ℃/15 ℃ for 90 days,and the germination rate could reach 60.2％.The content of soluble sugar and soluble protein decreased significantly during germination.The endogenous hormones GA3,brassinosterol(BR),zeanoside(ZR),indole acetic acid(IAA)and jasmonic acid(JA)synergically promoted seed germination and emergence,while ABA inhibited seed germination.
关键词
焕镛木/生理休眠/萌发特性Key words
Wooyoungia septentrionalis/physiological dormancy/germination characteristics引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据