The Effect of Colchicine Treatment on the Germination and Seedling Growth of Origanum vulgare L.
In order to explore the effects of colchicine treatment on Origanum vulgare L.seed germination and seedling growth,using Origanum vulgare L.seeds as research materials,Origanum vulgare L.seeds were treated with different concentrations(0(ck),0.01％,0.05％,0.10％,0.50％),and the physiological and biochemical indexes of Origanum vulgare L.seeds at germination stage and seedling growth stage were deter-mined respectively.The results showed that with the increase of colchicine concentration,the germination rate and germination potential of Origanum vulgare L.seeds increased first and then decreased.Under the treat-ment of 0.05％colchicine,the germination potential and germination rate of Origanum vulgare L.seeds reached the highest value,which was 1.3 times higher than that of the control.The seed germination was inhibited by 0.50％colchicine treatment.With the increase of colchicine concentration,the root length of Origanum vulgare L.seedlings gradually decreased,and the root thickness and leaf thickness gradually increased.Under 0.01％colchicine treatment,plant height,leaf length,leaf width and leaf area of Origanum vulgare L.seedlings reached the maximum value.With the increase of colchicine concentration,plant height and leaf width of Origanum vulgare L.seedlings decreased,POD activity increased first and then decreased,and soluble protein and soluble sugar contents were higher than ck,reaching the highest value at 0.01％.According to the membership function analysis of Origanum vulgare L.seed determination indics,the aver-age membership function value under 0.01％colchicine ranked first.The results showed that soaking 0.01％colchicine for 24 hours was the best treatment to promote seed germination and seedling growth of Origanum vulgare L..
Origanum vulgare L.colchicineseed germinationseedling growthphysiological index
万方数据
维普
