Study on Callus Induction and Its Growth and Development of in Vitro Plantlets of Medicago polymorpha L.
In order to investigate the callus induction and the growth of in vitro seedlings of 3 varieties of Medicago polymorpha L.(Shanghai,Daye,Zhongye),the sterile system,callus induction and proliferation of in vitro seedlings and related physiological and biochemical indexes were studied.The results showed that the best disinfection method for the three varieties of Medicago polymorpha L.aseptic system was 75％alcohol for 1 minutes+0.1％mercury bichloride from 10 to 15 minutes.Under light conditions,MS+KT 0.5 mg/L+2,4-D 2 mg/L was beneficial to increase the contents of soluble protein and soluble sugar,as well as the activities of POD,SOD and CAT in the induction process of cotyl callus.The content of MDA in the callus induction process of the cotyl decreased,which was the best medium for callus induction of 3 varieties of Medicago polymorpha L..Under dark conditions,MS+6-BA 2 mg/L+KT 2 mg/L+2,4-D 0.5 mg/L+NAA 0.5 mg/L could improve the contents of soluble protein and soluble sugar,and the activities of POD,SOD and CAT during callus proliferation,and reduce the MDA during callus proliferation,which was the best medium for the proliferation of callus of 3 varieties of Medicago polymorpha L..Under the light condition,MS+6-BA 1 mg/L+NAA 0.2 mg/L+chito-oligosaccharides(COS)0.5 mg/L and MS+KT 1 mg/L+NAA 0.2 mg/L+COS 0.5 mg/L could improve the contents of soluble protein and soluble sugar of the seedlings,of which the POD,SOD and CAT activities decreased the content of MDA in test tube seedlings,and it was a better medium for the growth and development of three varieties of Medicago polymorpha L.seedlings.
Medicago polymorpha L.sterile systeminduction of callusin vitro seedlingsgrowth and development
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