Induction of Loose Embryogenic Callus of Polyploid Arundo donax L.
In order to establish an efficient system of somatic embryogenesis of Arundo donax L.,the young stem segments of polyploid Arundo donax L.tissue culture seedlings with interganglionic seg-ments were selected as explants,and the loose embryogenic callus was induced by medium containing different hormone types and concentrations under the conditions of multiple subgenerations.The results showed that MS+0.25 mg/L 6-BA+1 mg/L 2,4-D was the best medium for inducing loose callus of Arundo donax L..It had the characteristics of fast callus induction,loose structure,soft texture and white and green color.The best medium for inducing loose embryogenic callus was 1/2 MS+0.50 mg/L 6-BA+0.50 mg/L 2,4-D.The embryogenic callus showed loose structure,hard tex-ture and fast growth by electron microscopy.The induced loose embryoid callus was transferred on hormone-free MS medium,and 5 subcultures were needed to obtain well-developed embryoid.
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