摘要
本研究以藏产卷丹百合的地下鳞茎为实验材料,采用不同接种方式、不同部位的鳞片及不同生长年限的鳞片,探索繁殖系数较高的离体快繁体系.结果表明,采用卷丹百合内层鳞片的芽诱导效果较好,且污染率较低,最佳接种方式为近轴面朝下,可显著地提高芽诱导数量,纵划处理有利于鳞片的芽诱导,三年生鳞片的诱导效果较好,所诱导的不定芽更加粗壮,长势好.最佳的鳞片诱导培养基为1.5 mg/L 6-BA和0.5 mg/L NAA,芽的平均数量较多,为11.898个;最佳的芽增殖培养基为2 mg/L 6-BA和0.5 mg/L NAA;添加0.5 mg/L IBA有利于芽苗生根,生根率较高,根粗壮,且根系较发达.将幼苗根植于V泥炭土∶V蛭石∶V珍珠岩=3∶1∶0.5的混合基质,成活率达到97.27％.
Abstract
In this study,the underground bulbs of Lilium lancifolium from Xizang were used as exper-imental materials,and the scales of different inoculation methods,different parts and different growth years were used to explore the in vitro rapid propagation system with high reproduction coeffi-cient.The results showed that the bud induction effect of inner scale of Lilium lancifolium was better and the contamination rate was lower.The optimal inoculation method was adaxial plane down,which could significantly increase the number of bud induction.The longitudinal striating treatment was beneficial to the bud induction of scales.The effect of scale induction in three years of growth was better,and the induced adventitious buds were more robust and grew well.The optimal scale induc-tion medium was 1.5 mg/L 6-BA and 0.5 mg/L NAA,and the average number of buds was 11.898.The optimal bud growth medium was 2 mg/L 6-BA and 0.5 mg/L NAA.The addition of 0.5 mg/L IBA was conducive to the rooting of the sprouts,the rooting rate was higher,the roots were strong and the roots were more developed.The survival rate of seedlings was 97.27％when they were planted in a mixture of Vpeat soil to Vvermiculite to Vperlite=3∶1∶0.5.
国家科技期刊平台
NSTL
维普
万方数据