Study on Rapid Propagation in Vitro of Lilium lancifolium Bulbs from Xizang
In this study,the underground bulbs of Lilium lancifolium from Xizang were used as exper-imental materials,and the scales of different inoculation methods,different parts and different growth years were used to explore the in vitro rapid propagation system with high reproduction coeffi-cient.The results showed that the bud induction effect of inner scale of Lilium lancifolium was better and the contamination rate was lower.The optimal inoculation method was adaxial plane down,which could significantly increase the number of bud induction.The longitudinal striating treatment was beneficial to the bud induction of scales.The effect of scale induction in three years of growth was better,and the induced adventitious buds were more robust and grew well.The optimal scale induc-tion medium was 1.5 mg/L 6-BA and 0.5 mg/L NAA,and the average number of buds was 11.898.The optimal bud growth medium was 2 mg/L 6-BA and 0.5 mg/L NAA.The addition of 0.5 mg/L IBA was conducive to the rooting of the sprouts,the rooting rate was higher,the roots were strong and the roots were more developed.The survival rate of seedlings was 97.27％when they were planted in a mixture of Vpeat soil to Vvermiculite to Vperlite=3∶1∶0.5.
万方数据
维普
