Study on the Differential Proteomics of Endosperm during the Germination of Tilia miqueliana Seeds
Tilia miqueliana seeds exhibit deep dormancy characteristics typical of woody plant species,which can be effectively broken through combined H2SO4 and GA3 treatment followed by cold stratifi-cation.To investigate endosperm alterations and metabolic dynamics during seed germination,pro-teomic and bioinformatics approaches were employed to analyze treated seeds subjected to H2SO4+GA3+cold stratification.The results showed that mass spectrometry identified 16 significantly altered proteins primarily associated with storage mobilization activity,programmed cell death(PCD),and redox reactions.It was revealed distinct biological process was mainly PCD before dormancy releasing while cellular catabolism became prominent post-dormancy after dormancy releasing.Four protein ag-gregation pathways showed marked activation:glycolysis/gluconeogenesis,secondary metabolite bio-synthesis,amino acid biosynthesis and glyoxylate metabolism.Following dormancy termination,stress response mechanisms dominated biological processes,accompanied by enhanced metabolic and amino acid synthesis pathways.Differential protein interaction network analysis constructed a network containing 25 nodes and 159 edges.Weighted Gene Co-expression Network Analysis(WGCNA)methodology established protein co-expression networks,with Gene Ontology(GO)functional and pathway annotation indicating their involvement in biological processes including photosynthesis,glu-cose metabolism,and metal ion responses.Furthermore,these networks participated in phytohor-mone biosynthesis and carbohydrate biogenesis pathways.
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