The Applied on Phenotypic Traits and Molecular Markers in DUS Testing of Hordeum vulgare
In order to explore the application of two methods,namely DUS phenotypic traits and SSR molecu-lar markers,in the DUS test of Hordeum vulgare,31 Hordeum vulgare varieties were used as materials and analyzed based on the phenotypic traits and molecular markers in the DUS test guidelines of Hordeum vul-gare.The results showed that molecular detection divided 31 Hordeum vulgare varieties into 34 pairs of simi-lar variety combinations.Cluster analysis indicated that the 31 Hordeum vulgare varieties could be divided in-to two major categories;With the increase in the number of differentially expressed sites in molecular detec-tion,the number of differentially expressed traits tested by DUS also increased.The codes of the same variety could vary from year to year.The average number of differential traits for each variety was 2.5,mainly con-centrated on the QN trait.The codes for the presence or absence and intensity of anthocyanin coloration could vary among different replicates or different years.
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