In this paper,gold nanoclusters (AuNCs)fluorescent probes with red fluorescence were prepared by using double protein as template,with BSA and FIC as reducing agent and stabilizer.The effects of different re-actant concentration,reaction time,reaction temperature and the amount of NaOH on the preparation of AuNCs were investigated.The UV spectra,infrared spectra,circular dichroism spectra and fluorescence spectra of the samples before and after the reaction were analyzed,and the formation of AuNCs fluorescent probes was confirmed. The experimental results show that the fluorescence intensity of the probe is stable in the buffer solution of pH3～pH12,and it also has good stability in the range of 20～400 mM NaCl solution.
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